Antibodies : a laboratory manual / edited by Edward A. Greenfield.

Second edition.
Cold Spring Harbor, New York : Cold Spring Harbor Laboratory Press, 2014.
xxi, 847 pages : illustrations ; 29 cm


Related name
Laboratory Manual
Summary note
"The focus of Antibodies: A Laboratory Manual, 2nd Edition, will be unchanged from the original edition by Ed Harlow and David Lane and will cover both the production and use of antibodies in a way that is accessible to the nonimmunologist. The emphasis will be on contemporary, essential antibody-based methods that are tried, true, necessary, and useful to a broad population of life scientists. The manual will provide up-to-date protocols that work reproducibly, along with explanations as to how and why methods work and how to choose between alternative approaches. Methods that have become research staples since the manual was originally published will be included at the same level of detail and organization as the existing topics"-- Provided by publisher.
Bibliographic references
Includes bibliographical references and index.
Machine generated contents note: -- Preface -- 1 Antibody Production by the Immune System -- Stefanie Sarantopoulos -- 2 The Antibody Molecule -- Stefanie Sarantopoulos -- 3 Antibody-Antigen Interactions -- Stefanie Sarantopoulos -- 4 Antibody Responses -- Stefanie Sarantopoulos -- 5 Selecting the Antigen -- Edward A. Greenfield, James DeCaprio, and Mohan Brahmandam -- 6 Immunizing Animals -- Edward A. Greenfield -- 7 Generating Monoclonal Antibodies -- Edward A. Greenfield -- 8 Growing Hybridomas -- Edward A. Greenfield -- 9 Characterizing Antibodies -- Frances Weis-Garcia and Robert H. Carnahan -- 10 Antibody Purification and Storage -- Jordan B. Fishman and Eric A. Berg -- 11 Engineering Antibodies -- James Dasch and Amy Dasch -- 12 Labeling Antibodies -- Eric A. Berg and Jordan B. Fishman -- 13 Immunoblotting -- Larisa Litovchick -- 14 Immunoprecipiation -- James DeCaprio and Thomas O. Kohl -- 15 Immunoassays -- Thomas O. Kohl and Carl A. Ascoli -- 16 Cell Staining -- Scott J. Rodig -- 17 Antibody Screening using High Throughput Flow Cytometry -- Thomas D.l. Duensing and Susan R. Watson -- Appendix I: Electrophoresis -- Appendix II: Protein Techniques -- Appendix III: General Information -- Appendix IV: Bacterial Expression -- Appendix V: Cautions -- Index.
Other editions
Revision of: Harlow, Edward. Using antibodies Cold Spring Harbor, N.Y. : Cold Spring Harbor Laboratory Press, c1999
  • 9781936113804 (hardback)
  • 1936113805 (hardback)
  • 9781936113811 (paper)
  • 1936113813 (paper)
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Supplementary Information