LC-MS/MS in proteomics : methods and applications / edited by Pedro R. Cutillas, John F. Timms.

Format
Book
Language
English
Published/​Created
New York, NY : Humana, c2010.
Description
x, 357 p. : ill. ; 27 cm.

Details

Subject(s)
Related name
Series
Bibliographic references
Includes bibliographical references and index.
Contents
Part I. Overviews of Techniques and Approaches in LC-MS-Based Proteomics -- 1. Approaches and Applications of Quantitative LC-MS for Proteomics and Activitomics / John F. Timms -- 2. Overview of Quantitative LC-MS Techniques for Proteomics and Activitomics / Pedro R. Cutillas -- 3. Instrumentation for LC-MS/MS in Proteomics / Robert Chalkley -- 4. Bioinformatics for LC-MS/MS-Based Proteomics / Richard J. Jacob -- 5. Analysis of Post-translational Modifications by LC-MS/MS / Claire E. Eyers -- Part II. Structural Analysis of Post-Translational Modifications -- 6. In-Depth Analysis of Protein Phosphorylation by Multidimensional Ion Exchange Chromatography and Mass Spectrometry / Pedro R. Cutillas -- 7. Mapping of Phosphorylation Sites by LC-MS/MS / Bernd Bodenmiller -- 8. Analysis of Carbohydrates on Proteins by Offline Normal-Phase Liquid Chromatography MALDI-TOF-MS/MS / Elaine Stephens -- Part III. Techniques for Quantitative LC-MS -- 9. Selected Reaction Monitoring Applied to Quantitative Proteomics / Bruno Domon -- 10. Basic Design of MRM Assays for Peptide Quantification / Claus Jorgensen -- 11. Proteome-Wide Quantitation by SILAC / Blagoy Blagoev -- 12. Quantification of Proteins by iTRAQ / Richard D. Unwin -- 13. Quantification of Proteins by Label-Free LC-MS/MS / Sabine Bahn -- Part IV. Analysis of Protein Complexes And Organelles -- 14. Protocol for Quantitative Proteomics of Cellular Membranes and Membrane Rafts / Ritchie Williamson -- 15. Organelle Proteomics by Label-Free and SILAC-Based Protein Correlation Profiling / Jens S. Andersen -- 16. Mapping Protein-Protein Interactions by Quantitative Proteomics / Blagoy Blagoev -- Part V. Analysis of Biological Fluids And Clinical Samples -- 17. Analysis of Serum Proteins by LC-MS/MS / Eithne Costello -- 18. Urinary Proteome Profiling Using 2D-DIGE and LC-MS/MS / Mark E. Weeks -- 19. Analysis of Peptides in Biological Fluids by LC-MS/MS / Pedro R. Cutillas -- Part VI. Novel Applications of LC-MS -- 20. Quantification of Protein Kinase Activities by LC-MS / Pedro R. Cutillas -- 21. A Protocol for Top-Down Proteomics Using HPLC and ETD/PTR-MS / Sarah R. Hart.
ISBN
  • 9781607617792
  • 160761779X
LCCN
2010930850
OCLC
646113672
RCP
C - S