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Immunoelectron microscopy : methods and protocols / edited by Steven L. Schwartzbach, Testuaki Osafune.
Format
Book
Language
English
Published/Created
New York : Springer, [2010], ©2010.
Description
xi, 352 pages : illustrations.
Availability
Available Online
Online Content
Online Content
Springer Nature - Complete Protocols
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Status
Location Service
Notes
ReCAP - Remote Storage
QH506 .M56 v.657
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Details
Related name
Schwartzbach, Steven D.
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Osafune, Tetsuaki
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Series
Methods in molecular biology ; v. 657.
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Methods in molecular biology ; 657
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Summary note
"Immunoelectron microscopy is a key technique that bridges the information gap between biochemistry, molecular biology, and ultrastructural studies placing macromolecular functions within a cellular context. In Immunoelectron Microscopy: Methods and Protocols, expert researchers combine the tools of the molecular biologist with those of the microscopist. From the molecular biology toolbox, this volume presents methods for antigen production by protein expression in bacterial cells, methods for epitope tagged protein expression in plant and animal cells allowing protein localization in the absence of protein specific antibodies as well as methods for the production of anti-peptide, monoclonal, and polyclonal antibodies. From the microscopy toolbox, sample preparation methods for cells, plant, and animal tissue are presented. Both cryo-methods, which have the advantage of retaining protein antigenicity at the expense of ultrastructural integrity, as well as chemical fixation methods that maintain structural integrity while sacrificing protein antigenicity have been included, with chapters examining various aspects of immunogold labeling. Written in the highly successful Methods in Molecular Biology" series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls" "Authoritative and essential, Immunoelectron Microscopy: Methods and Protocols seeks to facilitate an increased understanding of structure function relationships"--BOOK JACKET.
Bibliographic references
Includes bibliographical references and index.
Contents
Part I. Molecular Toolbox
1. Protein Antigen Expression in Escherichia coli for Antibody Production / Sebastian Y. Bednarek
2. Expression of Epitope-Tagged Proteins in Plants / Takuya Furuichi
3. Expression of Epitope-Tagged Proteins in Arabidopsis Leaf Mesophyll Protoplasts / Sang-Dong Yoo
4. Transient Expression of Epitope-Tagged Proteins in Mammalian Cells / Elizabeth Sztul
5. Production and Purification of Polyclonal Antibodies / Kazutaka Miyatake
6. Production and Purification of Monoclonal Antibodies / Kazutaka Miyatake
7. Production of Antipeptide Antibodies / Shalini Gupta
8. Preparation of Colloidal Gold Particles and Conjugation to Protein A, IgG, F(ab')2, and Streptavidin / Sadaki Yokota
Part II. Microscopy Toolbox
9. Immunoelectron Microscopy of Chemically Fixed Developing Plant Embryos / Steven D. Schwartzbach
10. Pre-embedding Immunogold Localization of Antigens in Mammalian Brain Slices / Thomas Schikorski
11. Pre-embedding Immunoelectron Microscopy of Chemically Fixed Mammalian Tissue Culture Cells / Kuniaki Takata
12. Immunoelectron Microscopy of Cryofixed and Freeze-Substituted Plant Tissues / Yoshinobu Mineyuki
13. In Vivo Cryotechniques for Preparation of Animal Tissues for Immunoelectron Microscopy / Yasuhisa Fujii
14. Immunoelectron Microscopy of Cryofixed Freeze-Substituted Mammalian Tissue Culture Cells / Akira Sawaguchi
15. Immunoelectron Microscopy of Cryofixed Freeze-Substituted Saccharomyces cerevisiae / Martin W. Goldberg
16. High-Resolution Molecular Localization by Freeze-Fracture Replica Labeling / Toyoshi Fujimoto
17. Pre-embedding Electron Microscopy Methods for Glycan Localization in Chemically Fixed Mammalian Tissue Using Horseradish Peroxidase-Conjugated Lectin / Hayato Kawakami
18. Pre-embedding Nanogold Silver and Gold Intensification / Ryuichi Masaki
19. The Post-embedding Method for Immunoelectron Microscopy of Mammalian Tissues: A Standardized Procedure Based on Heat-Induced Antigen Retrieval / Shuji Yamashita
20. Double-Label Immunoelectron Microscopy for Studying the Colocalization of Proteins in Cultured Cells / Kuniaki Takata
21. Serial Section Immunoelectron Microscopy of Algal Cells / Steven D. Schwartzbach
22. Freeze-Etch Electron Tomography for the Plasma Membrane Interface / Nobuhiro Morone
23. Localization of rDNA at Nucleolar Structural Components by Immunoelectron Microscopy / Yasushi Sato
24. Immunogold Labelling for Scanning Electron Microscopy / Jindriska Fisevova
25. Horseradish Peroxidase as a Reporter Gene and as a Cell-Organelle-Specific Marker in Correlative Light-Electron Microscopy / Thomas Schikorski
26. Monitoring Rapid Endocytosis in the Electron Microscope via Photoconversion of Vesicles Fluorescently Labeled with FMI-43 / Thomas Schikorski.
Show 25 more Contents items
ISBN
9781607617822
160761782X
9781607617839 (e-book)
1607617838 (e-book)
OCLC
639187711
RCP
C - S
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Supplementary Information
Other versions
Immunoelectron microscopy [electronic resource] : methods and protocols / edited by Steven L. Schwartzbach, Testuaki Osafune.
id
9962473533506421
Immunoelectron microscopy : methods and protocols / edited by Steven D. Schwartzbach, Testuaki Osafune.
id
9962224823506421
Immunoelectron Microscopy [electronic resource] : Methods and Protocols / edited by Steven D. Schwartzbach, Tetsuaki Osafune.
id
99125187203806421