In vitro mutagenesis protocols / edited by Jeff Braman.

3rd ed.
Totowa, NJ : Humana Press, c2010.
xiv, 442 p. : ill. (some col.) ; 27 cm.


MutagenesisMethodology [Browse]
Related name
Bibliographic references
Includes bibliographical references and index.
PART I. MUTAGENESIS IN VARIOUS MICROBIAL BACKGROUNDS -- 1. Mutagenesis Protocols in Saccharomyces cerevisiae by In Vivo Overlap Extension / Miguel Alcalde -- 2. In Vitro Mutagenesis of Brucella Species / Melissa Kahl-McDonagh -- 3. Random Mutagenesis Strategies for Campylobacter and Helicobacter Species / Arnoud H.M. van Vliet -- 4. Mutagenesis of the Repeat Regions of Herpesviruses Cloned as Bacterial Artifical Chromosomes / Venugopal Nair -- 5. An Efficient Protocol for VZV BAC-Based Mutagenesis / Hua Zhu -- 6. A Method for Rapid Genetic Integration into Plasmodium falciparum Utilizing Mycobacteriophage Bxbl Integrase / David A. Fidock -- PART II. PCR MUTAGENESIS -- 7. Random Mutagenesis by Error-Prone PCR / John C. Chaput -- 8. A Rapid and Versatile PCR-Based Site-Directed Mutagenesis Protocol for Generation of Mutations Along the Entire Length of a Cloned cDNA / Vincent Dammai -- 9. Rapid Sequence Scanning Mutagenesis Using In Silico Oligo Design and the Megaprimer PCR of Whole Plasmid Method (MegaWHOP) / Thorsten Eggert -- 10. Insertion and Deletion Mutagenesis by Overlap Extension PCR / Wang-Shick Ryu -- 11. Targeted Amplification of Mutant Strands for Efficient Site-Directed Mutagenesis and Mutant Screening / Qihan Dong -- 12. A Modified Inverse PCR Procedure for Insertion, Deletion, or Replacement of a DNA Fragment in a Target Sequence and Its Application in the Ligand Interaction Scan Method for Generation of Ligand-Regulated Proteins / Moti Liscovitch -- 13. Amplification of Orthologous Genes Using Degenerate Primers / Jim O. Vigoreaux -- PART III. REVIEWS -- 14. Computational Evaluation of Protein Stability Change upon Mutations / Nikolay V. Dokholyan -- 15. Approaches for Using Animal Models to Identify Loci That Genetically Interact with Human Disease-Causing Point Mutations / Josef D. Franke -- PART IV. PROTEIN EVOLUTION MUTAGENESIS -- 16. Using Peptide Loop Insertion Mutagenesis for the Evolution of Proteins / Kai Johnsson -- 17. Massive Mutagenesis®: High-Throughput Combinatorial Site-Directed Mutagenesis / Julien Sylvestre -- 18. Directed In Vitro Evolution of Reporter Genes Based on Semi-Rational Design and High - Throughput Screening / Zong-Ming Cheng -- 19. Ribosome Display for Rapid Protein Evolution by Consecutive Rounds of Mutation and Selection / Tetsuya Yomo -- PART V. PROTEIN STRUCTURE AND FUNCTION MUTAGENESIS -- 20. Fine-Tuning Enzyme Activity Through Saturation Mutagenesis / Holly H. Hogrefe -- 21. Characterization of Structural Determinants of Type 1 Corticotropin Releasing Hormone (CRH) Receptor Signalling Properties / Dimitris K. Grammatopoulos -- 22. Site-Directed Mutagenesis for Improving Biophysical Properties of VH Domains / Jamshid Tanha -- 23. Phenotype Based Functional Gene Screening Using Retrovirus-Mediated Gene Trapping in Quasi-Haploid RAW 264.7 Cells / Soon-Duck Ha -- 24. Site-Directed Disulfide Cross-Linking to Probe Conformational Changes of a Transporter During Its Functional Cycle: Escherichia coli AcrB Multidrug Exporter as an Example / Hiroshi Nikaido -- 25. Site-Specific Incorporation of Extra Components into RNA by Transcription Using Unnatural Base Pair Systems / Ichiro Hirao -- PART VI. RANDOM MUTAGENESIS -- 26. Mutagen™: A Random Mutagenesis Method Providing a Complementary Diversity Generated by Human Error-Prone DNA Polymerases / Hakim Kharrat -- 27. Random-Scanning Mutagenesis / Robert A. Smith -- 28. Easy Two-Step Method for Randomizing and Cloning Gene Fragments / Holly H. Hogrefe -- PART VII. MUTATOR BACTERIAL STRAIN MUTAGENESIS -- 29. Random Mutagenesis Using a Mutator Strain / Ranjan Sen -- 30. En Passant Mutagenesis: A Two Step Markerless Red Recombination System / Nikolaus Osterrieder.
  • 9781607616511
  • 1607616513
C - S