Protein blotting and detection : methods and protocols / edited by Biji T. Kurien, R. Hal Scofield.

New York : Humana Press, c2009.
xiii, 588 p. : ill.


Western immunoblotting [Browse]
Related name
Laboratory Manual
Methods in molecular biology, 1064-3745 ; 536
Bibliographic references
Includes bibliographical references and index.
1. Origins of Protein Blotting / Harry Towbin -- 2. Western Blotting: Remembrance of Past Things / W. Neal Burnette -- 3. Introduction to Protein Blotting / Biji T. Kurien and R. Hal Scofield -- 4. Simultaneous Immunoblotting Analysis with Activity Gel Electrophoresis and 2-D Gel Electrophoresis / Der-Yen Lee and Geen-Dong Chang -- 5. Diffusion Blotting for Rapid Production of Multiple Identical Imprints from Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis on a Solid Support / Ingrid Olsen and Harald G. Wiker -- 6. Tryptic Peptide Purification Using Polyvinylidene Difluoride Membrane for Matrix-Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry / Biji T. Kurien and R. Hal Scofield -- 7. Affinity Immunoblotting for Analysis of Antibody Clonotype Distribution in a Lupus Patient Developing Anti-Ro 60 Over Time / Biji T. Kurien and R. Hal Scofield -- 8. Non-electrophoretic Bi-directional Transfer of a Single SDS-PAGE Gel with Multiple Antigens to Obtain 12 Immunoblots / Biji T. Kurien and R. Hal Scofield -- 9. Slice Blotting / Graeme Lowe -- 10. Localizing Proteins by Tissue Printing / Rafael F. Pont-Lezica -- 11. Dot-Immunobinding Assay / S. Sumi, A. Mathai and V. V. Radhakrishnan -- 12. Capillary Blotting of Glycosaminoglycans on Nitrocellulose Membranes After Agarose Gel Electrophoresis Separation / Nicola Volpi and Francesca Maccari -- 13. Quantitative Computerized Western Blotting / Dalit Talmi-Frank, Charles L. Jaffe and Gad Baneth -- 14. Cationic Electrophoresis and Eastern Blotting / Engelbert Buxbaum -- 15. A Miniaturized Blotting System for Simultaneous Detection of Different Autoantibodies / Ulrich Canzler, Holger Bartsch, Kai Grossmann, Werner Lehmann, Karsten Conrad, Biji T. Kurien, Yaser Dorri, R. Hal Scofield and Michael Bachmann -- 16. Transfer and Multiplex Immunoblotting of a Paraffin Embedded Tissue / Joon-Yong Chung and Stephen M. Hewitt -- 17. Multistrip Western Blotting to Increase Quantitative Data Output / Anatoly Kiyatkin and Edita Aksamitiene -- 18. Western Blotting/Edman Sequencing using PVDF Membrane / Setsuko Komatsu -- 19. Blotting from PhastGel to Membranes by Ultrasound / Joseph Kost -- 20. Ultrarapid Electrophoretic Transfer of High and Low Molecular Weight Proteins Using Heat / Biji T. Kurien and R. Hal Scofield -- 21. Strip Immunoblotting of Multiple Antigenic Peptides to Nitrocellulose Membrane / Biji T. Kurien -- 22. Affinity Purification of Autoantibodies from an Antigen Strip Excised from a Nitrocellulose Protein Blot / Biji T. Kurien -- 23. Double-Blotting: A Solution to the Problem of Nonspecific Binding of Secondary Antibodies in Immunoblotting Procedures / Francoise Lasne -- 24. Efficient Electroblotting of Very Large Proteins Using a Vertical Agarose Electrophoresis System / Marion L. Greaser and Chad M. Warren -- 25. Semi-Dry Protein Transfer and Immunodetection of P-Selectin Using an Antibody to its C-terminal Tag / Padmaja Mehta -- 26. The Multiple Antigen Blot Assay: A Simple, Versatile and Multipurpose Immunoenzymatic Technique / Oscar Noya Sandra Losada, Marilyan Toledo and Belkisyole Alarcon de Noya -- 27. Blotting from Immobilized pH Gradient Gels: Application to Total Cell Lysates / Harry Towbin -- 28. Immunoprecipitation-Western Blot for Proteins of Low Abundance / Edward P. Trieu, Joanne K. Gross and Ira N. Targoff -- 29. Native Electrophoresis and Western Blot Analysis: Method and Applications / Ioannis N. Manoussopoulos and Mina Tsagris -- 30. Grid-Immunoblotting / Olga Yeliosof and R. Hal Scofield -- 31. Sequential Use of Immunoblots for Characterization of Autoantibody Specificities / Holger Bartsch and Michael Bachmann -- 32. Renaturation of Recombinant Ro 60 Autoantigen by Calcium Ions on PVDF Membrane / Biji T. Kurien and Michael Bachmann -- 33. Intermittent Microwave Irradiation Facilitates Antigen-Antibody Reaction in Western Blot Analysis / Yu-Ting Liu and Shinya Toyokuni -- 34. Detection of Protein-Protein Interactions by Far-Western Blotting / Kazuya Machida and Bruce J. Mayer -- 35. On-Membrane Tryptic Digestion of Proteins for Mass Spectrometry Analysis / Jose L. Luque-Garcia and Thomas A. Neubert -- 36. Western Blot Analysis of Adhesive Interactions under Fluid Shear Conditions: The Blot Rolling Assay / Robert Sackstein and Robert Fuhlbrigge -- 37. ELISPOT Assay on Membrane Microplates / Alexander E. Kalyuzhny -- 38. A Brief Review of Other Notable Protein Blotting Methods / Biji T. Kurien and R. Hal Scofield -- 39. Phosphoprotein Detection on Protein Electroblot Using a Phosphate-Specific Fluorophore / Lee Broderick Bockus and R. Hal Scofield -- 40. Rapid, Antibody-Free Detection of Recombinant Proteins on Blots Using Enzyme Fragment Complementation / Neil W. Charter, Joe Horecka, Chin-Yee Loh, Albert Doan, Tom Wehrman and Keith R. Olson -- 41. Fluorescent Labeling of Proteins and Its Application to SDS-PAGE and Western Blotting / F. Javier Alba, Salvador Bartolome, Antonio Bermudez and Joan-Ramon Daban -- 42. Whole and Strip Nitrocellulose Membrane as well as New Line Immunoblotting of Antigen Using the Chemiluminescence Technique / Yaser Dorri, Ali W. Khalili and R. Hal Scofield -- 43. Protein Stains to Detect Antigen on Membranes / Anil D'souza and R. Hal Scofield -- 44. Use of Nonradioactive Detection Method for North- and Southwestern Blot / Claudia Franke, Daniel Grafe, Holger Bartsch and Michael Bachmann -- 45. Immunoblotting Using Radiolabeled Reagents for Detection / Holger Bartsch, Claudia Franke and Michael Bachmann -- 46. Detection of Protein Carbonyls by Means of Biotin Hydrazide-Streptavidin Affinity Methods / Kenneth Hensley -- 47. "Rainbow" Western Blotting / Stan (Stanislaw) Krajewski -- 48. Multiple Antigen Detection Western Blotting / Stan Krajewski, Xianshu Huang and Maryla Krajewska -- 49. Detection of Calcium Binding by Ro 60 Multiple Antigenic Peptides on PVDF Membrane Using Quin-2 / Biji T. Kurien and Michael Bechamnn -- 50. Detection of La/SS-B by Western Blot Using Nanogold-Tagged Antibodies and Silver Enhancement / Shannon Maier, Sherry Hubbell and R. Hal Scofield -- 51. Imaging Systems for Westerns: Chemiluminescence vs. Infrared Detection / Suresh T. Mathews, Eric P. Plaisance and Teayoun Kim -- 52. A Fluorescent Codetection System for Immunoblotting and Proteomics Through ECL-Plex and CyDye Labeling / Ciara A. McManus, Pamela M. Donoghue and Michael J. Dunn -- 53. Visualization of Unstained Protein Bands on PVDF / Jun Park, Masaharu Mabuchi and Ajay Sharma -- 54. Improved Protein Detection Using Cold Microwave Technology / Aaron G. Smith, Jyothi Jayaram, Carol B. Johnson, E. Ann Ellis, Stanislav Vitha, Ellen W. Collisson and Andreas Holzenburg -- 55. TLC Blot (Far-Eastern Blot) and Its Applications / Takao Taki, Tania Valdes Gonzalez, Naoko Goto-Inoue, Takahiro Hayasaka and Mitsutoshi Setou -- 56. A Brief Review of Other Notable Protein Detection Methods on Blots / Biji T. Kurien and R. Hal Scofield -- 57. Sending Secret Messages on Nitrocellulose Membrane and the Use of a Molecular Pen for Orientation in ECL Membrane Assays / Biji T. Kurien.
  • 9781934115732 (hbk. : alk. paper)
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