Princeton University Library Catalog

Plant stress tolerance : methods and protocols / edited by Ramanjulu Sunkar.

Totowa, N.J. : Humana ; London : Springer [distributor], 2010.
xiv, 386 p. : ill. (some col.) ; 27 cm.
Summary note:
"A number of abiotic factors such as drought, salinity, extreme temperatures, low or high light intensity, and deficiency or toxic levels of nutrients have huge impacts on crop productivity, and a furthering of our understanding of the molecular, biochemical, and physiological basis of stress tolerance has been widely recognized as critical. In Plant Stress Tolerance: Methods and Protocols, expert researchers cover the most important widely-used techniques, including cutting-edge strategies, in a manner that ensures effective results. Beginning with reviews on dehydration, salinity, and cold tolerance as well as on oxidative stress, the volume then continues with methods involving topics such as describing the identification of stress-regulated genes, proteins, and microRNAs using diverse approaches, measurement of osmotic adjustment, proline levels, enzymes involved in proline metabolism, and sugars as well as determination of ROS levels, lipid peroxidation, ion leakage, and the enzymes involved in ROS detoxification. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introduction to to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls." "Comprehensive and up-to-date, Plant Stress Tolerance: Methods and Protocols provides a wide range of easy-to-follow protocols catering to the needs of plant physiologists, biochemists, and molecular biologists interested in probing this vital area of study."--BOOK JACKET.
Bibliographic references:
Includes bibliographical references and index.
PART I. Reviews and Overview Chapters -- 1. Dehydration Tolerance in Plana / Karen L. Kestor -- 2. Approaches to Identifying Genes for Salinity Tolerance and the Importance of Timescale / Rana Munns -- 3. Gene Regulation During Cold Stress Acclimation in Plants / Ramanjulu Sunkar -- 4. Redox-Dependent Regulation, Redox Control and Oxidative Damage in Plant Cells Subjected to Abiotic Stress / Karl-Josef Dietz -- 5. Array Platforms and Bioinformatics Tools for the Analysis of Plant Transcriptome in Response to Abiotic Stress / Mare Strickert -- 6. Metabolic Engineering of Glyoxalase Pathway for Enhancing Stress Tolerance in Plants / Sudbir K. Sopory -- PART II. Genome-Wide Approaches for Identification of Stress-Regulated Genes, Proteins, and Small RNAs -- 7. Genetic Screens to Identify Plant Stress Genes / Lasslo Sgabados -- 8. Arabidepsis Tiling Array Analysis to Identify the Stress-Responsive Genes / Motoaki Seki -- 9. Identification of Stess Responsive Genes in Plants Using Suppression Subtraction Hybridization: Ozone Stress as an Example / Ramamurthy Mabalingam -- 10. Identification of DNA-Binding Proteins and Protein-Protein Interactions by Yeast One-Hybrid and Yeast Two-Hybrid Screen / Karl-Josef Dietz -- 11. Functional Characterization of Water-Deficit Stress Responsive Genes Using RNAi / Kirankumar S. Mysore -- 12. Difference Gel Electrophoresis as a Tool to Discover Stress-Regulated Proteins / Jenny Renaut -- 13. Thiol-Disulfide Redox Proteomics in Plant Research / Elke Strober -- 14. Cloning of Stress-Responsive MicroRNAs and other Small RNAs from Plants / Ramanjulu Sunkar -- 15. An Array Platform for Identification of Stress-Responsive MicroRNAs in Plants / Guiliang Tang -- PART III. Antioxidant Enzymes and Metabolites -- 16. Spectrophotometry Assays for Antioxidant Enzymes in Plants / Bjorn Martin -- 17. Affinity Purification and Determination of Enzymatic Activity of Recombinantly Expressed Aldehyde Dehydrogenases / Horst Rohrig -- 18. Determination and Detection of Reactive Oxygen Species (ROS), Lipid Peroxidation, and Electrolyte leakage in Plants / Niranjani Jambunathan -- PART IV. Osmotic Adjustment and Ion Measurements -- 19. Quantification of Water Stress-Induced Osmotic Adjustment and Proline Accumulation for Arabidopsis thaliana Molecular Genetic Studies / Paul E. Verslues -- 20. Methods for Determination of Proline in Plants / Laszlo Szabados -- 21. A New Method for Accurately Measuring δ1-Pyrroline-5-Carboxylate Synthetase Activity / Arnould Savoure -- 22. Extraction and Analysis of Soluble Carbohydrates / Niels Maness -- 23. Measuring Soluble Ion Concentrations (Na+, K+, Cl-) in Salt-Treated Plants / Timothy D. Colmer.
  • 9781607617013 (hbk.)
  • 1607617013 (hbk.)
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