Princeton University Library Catalog

Hepatocytes : methods and protocols / edited by Patrick Maurel.

Totowa, N.J. : Humana ; London : Springer [distributor], 2010.
xix, 553 p. : ill. ; 27 cm.
Summary note:
"Hepatocytes account for approximately 80% of the liver mass and play a significant role in various aspects of liver physiopathology, exhibiting unrivaled complexity and diversity of functions. In Hepatocytes: Methods and Protocols, expert researchers provide the reader with methods, technical protocols, and review chapters focusing on selected areas of hepatocyte biology including isolation, culture, differentiation and stem cells, and hepatocyte use in clinical, basic, and applied research. With a specific emphasis on human hepatocytes, the volume presents chapters covering subjects including hepatocyte culture models, cryopreservation methods, differentiation assessment, liver ontogenesis, production of hepatocytes from stem cells, drug/xenobiotic metabolism, toxicity and transport, bile acid and blood coagulation factor production, infection by HBV and HCV, humanized animals, biortificial liver devices, hepatocyte transplantation As a volume in the highly successful Methods in Molecular Biology" series, protocol Chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls." "Comprehensive and cutting-edge, Hepatocytes: Methods and Protocols will be useful to all those who are currently using or planning to use human, or animal, hepatocytes to investigate any aspect of liver physiopathology or who are interested in liver development or liver stem cells and liver biotherapy."--BOOK JACKET.
Bibliographic references:
Includes bibliographical references and index.
1. General Review on In Vitro Hepatocyte Models and Their Applications / Andre Guillouzo -- 2. Human Foetal Hepatocytes: Isolation, Characterization, and Transplantation / Dominique Mahieu-Caputo -- 3. Isolation and Culture of Primary Hepatocytes from Resected Human Liver Tissue / Eliane Alexandre -- 4. Optimisation of the Cryopreservation of Primary Hepatocytes / Nicola J. Hewitt -- 5. Cryopreservation of Human Hepatocytes for Clinical Use / Robin D. Hughes -- 6. Hepatocyte Differentiation / Curtis J. Omiecinski -- 7. Reversible Manipulation of Apoptosis Sensitivity in Cultured Hepatocytes by Matrix-Mediated Manipulation of Signaling Activities / Jan G. Hengstler -- 8. Markers arid Signaling Factors for Stem Cell Differentiation to Hepatocytes: Lessons from Developmental Studies / Frederic Lemaigre -- 9. Hepatic Stem Cells / Catherine Verfaillie -- 10. Hepatic Stem Cells and Liver Developments / Naoya Kobayashi -- 11. Generation of Hepatocytes from Human Embryonic Stem Cells / Neta Lavon -- 12. Isolation and Culture of Adult Human Liver Progenitor Cells: In Vitro Differentiation to Hepatocyte-Like Cells / Martine Daujat-Chavanieu -- 13. The HepaRG Cell Line: Biological Properties and Relevance as a Tool for Cell Biology, Drug Metabolism, and Virology Studies / David Durantel -- 14. Transdifferentiation of Pancreatic Cells to Hepatocytes / David Tosh -- 15. Evaluation of Drug Metabolism, Drug-Drug Interactions, and In Vitro Hepatotoxicity with Cryopreserved Human Hepatocytes / Albert P. Li -- 16. The Use of Human Hepatocytes to Investigate Drug Metabolism and CYP Enzyme Induction / Gerard Fabre -- 17. The Use of Hepatocytes to Investigate UDP-Glucuronosyltransferases and Sulfotransferases / Jacques Magdalou -- 18. The Use of Hepatocytes to Investigate Drug Uptake Transporters / Yuichi Sugiyama -- 19. Metabonomic Studies on Human Hepatocyte in Primary Culture / Bernard Walther -- 20. The Application of HepRG Cells in Evaluation of Cytochrome P450 Induction Properties of Drug Compounds / Tommy B. Andersson -- 21. The Use of Hepatocytes to Investigate Drug Toxicity / Maria Teresa Donato -- 22. The Use of Human Hepatocytes to Investigate Bile Acid Synthesis / Lisa-Mari Nilsson -- 23. Use of Human Hepatocytes to Investigate Blood Coagulation Factor / Patrick Maurel -- 24. Use of Human Hepatocytes to Investigate HCV Infection / Patrick Maurel -- 25. The Use of Hcpatocytes to Investigate HDV Infection: The HDV/HcpaRG Model / Camille Sureau -- 26. Rodent Models of Liver Repopulation / Helene Gilgenkrantz -- 27. Chimeric Mice with Humanized Liver: Tools for the Study of Drug Metabolism, Excretion, and Toxicity / Markus Grompe -- 28. Bioartificial Liver Support Systems / Gesine Pless -- 29. Human Hepatocyte Transplantation / Ira J. Fox.
  • 9781607616870 (hbk.)
  • 1607616874 (hbk.)
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