Cyclooxygenases : methods and protocols / edited by Samir S. Ayoub, Roderick J. Flower, Michael P. Seed.

Format
Book
Language
English
Published/​Created
New York, NY : Humana Press, c2010.
Description
xi, 254 p. : ill. (some col.) ; 27 cm.

Details

Subject(s)
Cyclooxygenases [Browse]
Related name
Form/​Genre
Laboratory Manual
Series
Bibliographic references
Includes bibliographical references and index.
Contents
Part I. In Vitro Protocols for Studying Expression and Activi ty of Cyclooxygenase Enzymes and Characterisation of Their Inhibition Patterns -- 1. In Vitro Cyclo-oxygenase Expression and Activity Protocols: Introduction to Part I / Roderick J. Flower -- 2. Techniques Used to Study Regulation of Cyclooxygenase-2 Promoter Sites / Hiroyasu Inoue and Rieko Nakata -- 3. Purification of Recombinant Human COX-1 and COX-2 / James K. Gierse -- 4. Cloning and Expression of Cyclooxygenase-1 and Cyclooxygenase-2 / Nicholas R. Staten and Beverly A. Reitz -- 5. Expression of Cyclooxygenase Isoforms in the Baculovirus Expression System / John C. Hunter, Natalie M. Myres, and Daniel L. Simmons -- 6. Peroxidase Active Site Activity Assay / Kelsey C. Duggan, Joel Musee, and Lawrence J. Marnett -- 7. Study of Inhibitors of the PGH Synthases for which Potency Is Regulated by the Redox State of the Enzymes / Olivier Boutaud and John A. Oates -- 8. Different Methods for Testing Potential Cyclooxygenase-1 and Cyclooxygenase-2 Inhibitors / Stefan Laufer and Sabine Luik -- 9. In Vitro Cyclooxygenase Activity Assay in Tissue Homogenates / Samir S. Ayoub -- 10. Techniques Used to Characterize the Binding of Cyclooxygenase Inhibitors to the Cyclooxygenase Active Site / William F. Hood -- 11. In Vitro Assays for Cyclooxygenase Activity and Inhibitor Characterization / Mark C. Walker and James K. Gierse -- -- Part II. Extraction and Measurement of Prostanoids and Isoprostanes -- 12. Extraction and Measurement of Prostanoids and Isoprostanes: Introduction to Part II / Paola Patrignani -- 13. Prostanoid Extraction and Measurement / Lorenzo Polenzani and Samir S. Ayoub -- 14. Measurement of 8-Iso-Prostaglandin F2a in Biological Fluids as a Measure of Lipid Peroxidation / Stefania Tacconelli, Marta L. Capone, and Paola Patrignani -- -- Part III. In Vivo Models to Study Involve ment of Cyclooxygenase Products in Health and Disease -- 15. In Vivo Models to Study Cyclooxygenase Products in Health and Disease: Introduction to Part III / Derek W. Gilroy, Melanie Stables, and Justine Newson -- 16. Protocols to Assess the Gastrointestinal Side Effects Resulting from Inhibition of Cyclo-Oxygenase Isoforms ./ Brendan J.R. Whittle -- 17. Cyclooxygenase Enzymes and Their Products in the Carrageenan-Induced Pleurisy in Rats / Adrian R. Moore, Samir S. Ayoub, and Michael P. Seed -- 18. Iloprost-Induced Nociception: Determination of the Site of Anti-nociceptive Action of Cyclooxygenase Inhibitors and the Involvement of Cyclooxygenase Products in Central Mechanisms of Nociception / Samir S. Ayoub and Regina M. Botting
ISBN
  • 9781588299536 (hbk. : alk. paper)
  • 1588299538 (hbk. : alk. paper)
  • 9781597453646 (e-ISBN)
  • 1597453641 (e-ISBN)
OCLC
618851309
RCP
C - S