Princeton University Library Catalog

Cyclooxygenases : methods and protocols / edited by Samir S. Ayoub, Roderick J. Flower, Michael P. Seed.

Format:
Book
Language:
English
Published/​Created:
New York, NY : Humana Press, c2010.
Description:
xi, 254 p. : ill. (some col.) ; 27 cm.
Series:
Bibliographic references:
Includes bibliographical references and index.
Contents:
Part I. In Vitro Protocols for Studying Expression and Activi ty of Cyclooxygenase Enzymes and Characterisation of Their Inhibition Patterns -- 1. In Vitro Cyclo-oxygenase Expression and Activity Protocols: Introduction to Part I / Roderick J. Flower -- 2. Techniques Used to Study Regulation of Cyclooxygenase-2 Promoter Sites / Hiroyasu Inoue and Rieko Nakata -- 3. Purification of Recombinant Human COX-1 and COX-2 / James K. Gierse -- 4. Cloning and Expression of Cyclooxygenase-1 and Cyclooxygenase-2 / Nicholas R. Staten and Beverly A. Reitz -- 5. Expression of Cyclooxygenase Isoforms in the Baculovirus Expression System / John C. Hunter, Natalie M. Myres, and Daniel L. Simmons -- 6. Peroxidase Active Site Activity Assay / Kelsey C. Duggan, Joel Musee, and Lawrence J. Marnett -- 7. Study of Inhibitors of the PGH Synthases for which Potency Is Regulated by the Redox State of the Enzymes / Olivier Boutaud and John A. Oates -- 8. Different Methods for Testing Potential Cyclooxygenase-1 and Cyclooxygenase-2 Inhibitors / Stefan Laufer and Sabine Luik -- 9. In Vitro Cyclooxygenase Activity Assay in Tissue Homogenates / Samir S. Ayoub -- 10. Techniques Used to Characterize the Binding of Cyclooxygenase Inhibitors to the Cyclooxygenase Active Site / William F. Hood -- 11. In Vitro Assays for Cyclooxygenase Activity and Inhibitor Characterization / Mark C. Walker and James K. Gierse -- -- Part II. Extraction and Measurement of Prostanoids and Isoprostanes -- 12. Extraction and Measurement of Prostanoids and Isoprostanes: Introduction to Part II / Paola Patrignani -- 13. Prostanoid Extraction and Measurement / Lorenzo Polenzani and Samir S. Ayoub -- 14. Measurement of 8-Iso-Prostaglandin F2a in Biological Fluids as a Measure of Lipid Peroxidation / Stefania Tacconelli, Marta L. Capone, and Paola Patrignani -- -- Part III. In Vivo Models to Study Involve ment of Cyclooxygenase Products in Health and Disease -- 15. In Vivo Models to Study Cyclooxygenase Products in Health and Disease: Introduction to Part III / Derek W. Gilroy, Melanie Stables, and Justine Newson -- 16. Protocols to Assess the Gastrointestinal Side Effects Resulting from Inhibition of Cyclo-Oxygenase Isoforms ./ Brendan J.R. Whittle -- 17. Cyclooxygenase Enzymes and Their Products in the Carrageenan-Induced Pleurisy in Rats / Adrian R. Moore, Samir S. Ayoub, and Michael P. Seed -- 18. Iloprost-Induced Nociception: Determination of the Site of Anti-nociceptive Action of Cyclooxygenase Inhibitors and the Involvement of Cyclooxygenase Products in Central Mechanisms of Nociception / Samir S. Ayoub and Regina M. Botting
Subject(s):
Cyclooxygenases [Browse]
Form/​Genre:
Laboratory Manuals.
ISBN:
  • 9781588299536 (hbk. : alk. paper)
  • 1588299538 (hbk. : alk. paper)
  • 9781597453646 (e-ISBN)
  • 1597453641 (e-ISBN)
OCLC:
618851309
Related name:
RCP:
C - S