Protein chromatography : methods and protocols / edited by Dermot Walls and Sinéad T. Loughran.

Format
Book
Language
English
Published/​Created
New York, N.Y. : Humana Press, [2011], ©2011.
Description
x, 527 pages : illustrations (some color) ; 27 cm.

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    Details

    Subject(s)
    Series
    Bibliographic references
    Includes bibliographical references and index.
    Contents
    • A digest of protein purification
    • Gel-filtration chromatography
    • Immunoaffinity chromatography
    • Avoiding proteolysis during protein chromatography
    • Scale-up of protein purification : Downstream processing issues
    • Phage display : A powerful technology for the generation of high specificity affinity reagents from alternative immune sources
    • Engineering protein stability
    • Microfluidics in protein chromatography
    • Tagging recombinant proteins to enhance solubility and aid purification
    • Storage and lyophilisation of pure proteins
    • Differential precipitation and solubilization of proteins
    • Ion-exchange chromatography : Basic principles and application to the partial purification of soluble mammalian prolyl oligopeptidase
    • Protein quantitation and analysis of purity
    • Purification of proteins fused to glutathione S-transferase
    • Purification of proteins fused to maltose-binding protein
    • Purification of proteins from baculovirus-infected insect cells
    • Purification of poly-histidine-tagged proteins
    • Immobilized metal affinity chromatography/reversed-phase enrichment of phosphopeptides and analysis by cid/etd tandem mass spectrometry
    • Tag removal by site-specific cleavage of recombinant fusion proteins
    • Purification of antibodies using affinity chromatography
    • Optimized generation of high-affinity, high-specificity single-chain Fv antibodies from multiantigen immunized chickens
    • Measuring protein-protein interactions using biacore
    • Ultra-performance liquid chromatography-mass spectrometry of proteins
    • Hydrophobic interaction chromatography
    • Fast protein liquid chromatography
    • Shotgun proteomics : A qualitative approach applying isoelectric focusing on immobilized pH gradient and LC-MS/MS
    • Shotgun proteomics : A relative quantitative approach using off-gel electrophoresis and LC-MS/MS
    • Clinical proteomics : Liquid chromatography-mass spectrometry purification systems
    • Strategies for the purification of membrane proteins
    • A multi-step chromatographic strategy to purify three fungal endo-[beta]-glucanases.
    OCLC
    680057439
    RCP
    C - S
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