Mass spectrometry imaging : principles and protocols / edited by Stanislav S. Rubakhin, Jonathan V. Sweedler.

Totowa, N.J. : Humana ; London : Springer [distributor], 2010.
xiv, 487 p. : ill. (some col.) ; 27 cm.


Related name
  • Methods in molecular biology (Clifton, N.J.) ; v. 656. [More in this series]
  • Methods in molecular biology, 1064-3745 ; 656
Summary note
"Mass spectrometry (MS) offers unmatched capabilities for the detection, characterization, and identification of a broad range of analytes. Mass spectrometry imaging (MSI) integrates MS data with information on the spatial distributions of the analytes, further enhancing the applicability of MS. In Mass Spectrometry Imaging: Principles and Protocols, expert practitioners from academia, industry, and the Clinic contribute cutting-edge protocols describing the application of MSI to investigations of analyte localization in a variety of specimens, from microorganisms to plant and animal tissues. Divided into three sections, this volume presents the principles of MS, current and future trends of MSI, and qualitative and quantitative protocols to measure and identify endogenous metabolites and xenobiotics. An array of MSI approaches and technologies for characterizing peptide and protein distributions are described in detail. Written in the highly successful Methods in Molecular Biology™ series format, protocol chapters include introductions to their respective topics, lists of the necessary materials and reagents, and step-by-step, readily reproducible laboratory procedures. Also included are notes providing tips to avoid experimental pitfalls and helpful suggestions for method troubleshooting." "Comprehensive and up-to-date, Mass Spectrometry Imaging: Principles and Protocols is written for scientists, biological and chemical engineers, and clinicians who are interested in applying MSI in their work and those who would benefit from having detailed experimental guidelines available in a single, convenient source."--BOOK JACKET.
Bibliographic references
Includes bibliograhical references and index.
Part I. Introductory Reviews And Tutorials -- 1. Imaging Mass Spectrometry: Viewing the Future / Richard M. Caprioli -- 2. A Mass Spectrometry Primer for Mass Spectrometry Imaging / Jonathan V. Sweedler -- 3. Imaging of Metals, Metalloids, and Non-metals by Laser Ablation Inductively Coupled Plasma Mass Spectrometry (LA-ICP-MS) in Biological Tissues / J. Susanne Becker -- Part II. Protocols For MS Imaging of Distribution of Small Molecules Including Metabolites and Pharmaceuticals -- 4. Lipid Detection, Identification, and Imaging Single Cells with SIMS / Andrew G. Ewing -- 5. The Application and Potential of Ion Mobility Mass Spectrometry in Imaging MS with a Focus on Lipids / Shelley N. Jackson -- 6. Quantitative Imaging of Chemical Composition in Single Cells by Secondary Ion Mass Spectrometry: Cisplatin Affects Calcium Stores in Renal Epithelial Cells / Subhash Chandra -- 7. Imaging MALDI Mass Spectrometry of Sphingolipids Using an Oscillating Capillary Nebulizer Matrix Application System / M. Cameron Sullards -- 8. Mapping Pharmaceuticals in Rat Brain Sections Using MALDI Imaging Mass Spectrometry / Walter A. Korfmacher -- 9. Laser Ablation Electrospray Ionization for Atmospheric Pressure Molecular Imaging Mass Spectrometry / Akos Vertes -- 10. Matrix-Assisted Laser Desorption/Ionization and Nanoparticle-Based Imaging Mass Spectrometry for Small Metabolites: A Practical Protocol / Mitsutoshi Setou -- 11. Cellular Imaging Using Matrix-Enhanced and Metal-Assisted SIMS / Sander R. Piersma -- 12. Tandem Mass Spectrometric Methods for Phospholipid Analysis from Brain Tissue / Richard A. Yost -- 13. Chemical Imaging with Desorption Electrospray Ionization Mass Spectrometry / Gary J. Van Berkel -- 14. Mass Spectrometry Imaging of Small Molecules Using Matrix-Enhanced Surface-Assisted Laser Desorption/Ionization Mass Spectrometry (ME-SALDI-MS) / Lin He -- 15. Preparation of Single Cells for Imaging Mass Spectrometry / Kristen S. Kulp -- 16. Applying Imaging ToF-SIMS and PCA in Differentiation of Tissue Types / Kuang Jen J. Wu -- Part III. Prtocols for MS Imaging of Distribution of Peptides And Proteins -- 17. Direct Molecular Analysis of Whole-Body Animal Tissue Sections by MALDI Imaging Mass Spectrometry / Richard M. Caprioli -- 18. MALDI Direct Analysis and Imaging of Frozen Versus FFPE Tissues: What Strategy for Which Sample? / Isabelle Fournier -- 19. On Tissue Protein Identification Improvement by N-Terminal Peptide Derivatization / Isabelle Fournier -- 20. Specific MALDI-MSI: TAG-MASS / Isabelle Fournier -- 21. Structurally Selective Imaging Mass Spectrometry by Imaging Ion Mobility-Mass Spectrometry / Jeffrey R. Enders -- 22. Tutorial: Multivariate Statistical Treatment of Imaging Data for Clinical Biomarker Discovery / Detlev Suckau -- 23. Applications of MALDI-MSI to Pharmaceutical Research / Markus Stoeckli -- 24. Tissue Preparation for the In Situ MALDI MS Imaging of Proteins, Lipids, and Small Molecules at Cellular Resolution / Jeffrey N. Agar -- 25. Imaging of Similar Mass Neuropeptides in Neuronal Tissue by Enhanced Resolution MALDI MS with an Ion Trap-Orbitrap™ Hybrid Instrument / Maria C. Prieto Conaway -- 26. Mass Spectrometric Imaging of Neuropeptides in Decapod Crustacean Neuronal Tissues / Lingjun Li -- 27. Mass Spectrometry Imaging Using the Stretched Sample Approach / Jonathan V. Sweedler.
  • 9781607617457 (hbk.)
  • 1607617455 (hbk.)
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