Receptor signal transduction protocols / edited by Gary B. Willars and R.A. John Challiss.

Format
Book
Language
English
Published/​Created
New York : Springer, [2011], ©2011.
Description
xi, 479 pages : illustrations (some color) ; 27 cm.

Availability

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    Details

    Series
    Bibliographic references
    Includes bibliographical references and index.
    Contents
    • Inducible expression of G protein-coupled receptors in transfected cells / Beryl Koener and Emmanuel Hermans
    • Using the Flp-In T-Rex system to regulate GPCR expression / Richard J. Ward, Elisa Alvarez-Curto, and Graeme Milligan
    • Viral infection for GPCR expression in eukaryotic cells / Antonio Porcellini, Luisa Iacovelli, and Antonio De Blasi
    • Generation of epitope-tagged GPCRs / Yan Huang and Gary B. Willars
    • Use of site-directed mutagenesis to study GPCRs / Alex C. Conner ... [et al.]
    • Approaches to study GPCR regulation in native systems / Jonathon M. Willets
    • Heterologous expression of GPCRs in fission yeast / John Davey and Graham Ladds
    • Radioligand binding methods for membrane preparations and intact cells / David B. Bylund and Myron L. Toews
    • Quantification of GPCR mRNA using real-time RT-PCR / Trond Brattelid and Finn Olav Levy
    • Determining allosteric modulator mechanism of action : integration of radioligand binding and functional assay data / Christopher J. Langmead
    • Design and use of fluorescent ligands to study ligand-receptor interactions in single living cells / Stephen J. Briddon, Barrie Kellam, and Stephen J. Hill
    • Examining site-specific GPCR phosphorylation / Adrian J. Butcher, Andrew B. Tobin, and Kok Choi Kong
    • Ubiquitination of GPCRs / Adriana Caballero and Adriano Marchese
    • [35S]GTPgS binding as an index of total G-protein and Ga-subtype-specific activation by GPCRs / Rajendra Mistry, Mark R. Dowling, and R. A. John Challiss
    • Using calcium imaging as a readout of GPCR activation / Martin D. Bootman and H. Llewelyn Roderick
    • Measuring spatiotemporal dynamics of cyclic AMP signaling in real-time using FRET-based biosensors / Frank Gesellchen ... [et al.]
    • Determining the activation of Rho as an index of receptor coupling to G12/13 proteins / Michio Nakaya ... [et al.]
    • Use of translocating fluorescent biosensors for real-time monitoring of GPCR-mediated signaling events / Carl P. Nelson and R.A. John Challiss
    • Study of GPCR-protein interactions using gel overlay assays and glutathione-S-transferase-fusion protein pull-downs / Ashley E. Brady ... [et al.]
    • Study of GPCR-protein interactions by BRET / Martina Kocan and Kevin D.G. Pfleger
    • Time resolved FRET strategy with fluorescent ligands to analyze receptor interactions in native tissues : application to GPCR oligomerization / Martin Cottet ... [et al.]
    • Peptide affinity purification for the isolation and identification of GPCR-associated protein complexes / Pascal Maurice, Avais M. Daulat, and Ralf Jockers
    • Tandem affinity purification and identification of GPCR-associated protein complexes / Avais M. Daulat, Pascal Maurice, and Ralf Jockers
    • Identification of GPCR localization in detergent resistant membranes / Ranju Kumari and Anna Francesconi
    • Analysis of GPCR localization and trafficking / James N. Hislop and Mark von Zastrow
    • Statistical methods in research / Domenico Spina.
    ISBN
    • 1617791253
    • 9781617791253
    • 9781617791260 (e-isbn)
    • 1617791261 (e-isbn)
    OCLC
    706784502
    RCP
    C - S
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