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Receptor signal transduction protocols / edited by Gary B. Willars and R.A. John Challiss.
Format
Book
Language
English
Published/Created
New York : Springer, [2011], ©2011.
Description
xi, 479 pages : illustrations (some color) ; 27 cm.
Availability
Available Online
Online Content
Springer Nature - Complete Protocols
Copies in the Library
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Call Number
Status
Location Service
Notes
ReCAP - Remote Storage
QH506 .M56 v.746
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Related name
Willars, Gary B.
[Browse]
Challiss, R. A. J. (R. A. John)
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Series
Springer protocols (Series)
[More in this series]
Methods in molecular biology (Clifton, N.J.) ; v. 746.
[More in this series]
Methods in molecular biology, 1064-3745 ; v.746
Springer protocols
Bibliographic references
Includes bibliographical references and index.
Contents
Inducible expression of G protein-coupled receptors in transfected cells / Beryl Koener and Emmanuel Hermans
Using the Flp-In T-Rex system to regulate GPCR expression / Richard J. Ward, Elisa Alvarez-Curto, and Graeme Milligan
Viral infection for GPCR expression in eukaryotic cells / Antonio Porcellini, Luisa Iacovelli, and Antonio De Blasi
Generation of epitope-tagged GPCRs / Yan Huang and Gary B. Willars
Use of site-directed mutagenesis to study GPCRs / Alex C. Conner ... [et al.]
Approaches to study GPCR regulation in native systems / Jonathon M. Willets
Heterologous expression of GPCRs in fission yeast / John Davey and Graham Ladds
Radioligand binding methods for membrane preparations and intact cells / David B. Bylund and Myron L. Toews
Quantification of GPCR mRNA using real-time RT-PCR / Trond Brattelid and Finn Olav Levy
Determining allosteric modulator mechanism of action : integration of radioligand binding and functional assay data / Christopher J. Langmead
Design and use of fluorescent ligands to study ligand-receptor interactions in single living cells / Stephen J. Briddon, Barrie Kellam, and Stephen J. Hill
Examining site-specific GPCR phosphorylation / Adrian J. Butcher, Andrew B. Tobin, and Kok Choi Kong
Ubiquitination of GPCRs / Adriana Caballero and Adriano Marchese
[35S]GTPgS binding as an index of total G-protein and Ga-subtype-specific activation by GPCRs / Rajendra Mistry, Mark R. Dowling, and R. A. John Challiss
Using calcium imaging as a readout of GPCR activation / Martin D. Bootman and H. Llewelyn Roderick
Measuring spatiotemporal dynamics of cyclic AMP signaling in real-time using FRET-based biosensors / Frank Gesellchen ... [et al.]
Determining the activation of Rho as an index of receptor coupling to G12/13 proteins / Michio Nakaya ... [et al.]
Use of translocating fluorescent biosensors for real-time monitoring of GPCR-mediated signaling events / Carl P. Nelson and R.A. John Challiss
Study of GPCR-protein interactions using gel overlay assays and glutathione-S-transferase-fusion protein pull-downs / Ashley E. Brady ... [et al.]
Study of GPCR-protein interactions by BRET / Martina Kocan and Kevin D.G. Pfleger
Time resolved FRET strategy with fluorescent ligands to analyze receptor interactions in native tissues : application to GPCR oligomerization / Martin Cottet ... [et al.]
Peptide affinity purification for the isolation and identification of GPCR-associated protein complexes / Pascal Maurice, Avais M. Daulat, and Ralf Jockers
Tandem affinity purification and identification of GPCR-associated protein complexes / Avais M. Daulat, Pascal Maurice, and Ralf Jockers
Identification of GPCR localization in detergent resistant membranes / Ranju Kumari and Anna Francesconi
Analysis of GPCR localization and trafficking / James N. Hislop and Mark von Zastrow
Statistical methods in research / Domenico Spina.
Show 23 more Contents items
ISBN
1617791253
9781617791253
9781617791260 (e-isbn)
1617791261 (e-isbn)
OCLC
706784502
RCP
C - S
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Other versions
Receptor signal transduction protocols / edited by Gary B. Willars and R.A. John Challiss.
id
9967791083506421
Receptor signal transduction protocols [electronic resource] / edited by Gary B. Willars and R.A. John Challiss.
id
9967194073506421
Receptor Signal Transduction Protocols [electronic resource] : Third Edition / edited by Gary B. Willars, R.A. John Challiss.
id
99125204314106421