Microtubule dynamics : methods and protocols / edited by Anne Straube.

New York : Humana ; Springer, c2011.
xi, 319 p. : ill. ; 27 cm.


Microtubules [Browse]
Bibliographic references
Includes bibliographical references and index.
How to measure microtubule dynamics / Anne Straube -- Purification of tubulin from porcine brain / Christopher Gell ... [et al.] -- Purification of tubulin from the fission yeast Schizosaccharomyces pombe / Douglas R. Drummond ... [et al.] -- Generation of differentially polyglutamylated microtubules / Benjamin Lacroix and Carsten Janke -- Preparation of pure tyrosinated or detyrosinated tubulin isoforms / Laurence Lafanechere and Didier Job -- ab-tubulin and microtubule-binding assays / Jaime N. Campbell and Kevin C. Slep -- Analysis of microtubule assembly kinetics using turbidimetry / Felicia Gaskin -- Isolation of centrosomes from cultured cells / Simone Reber -- Preparation of dual-color polarity-marked fluorescent microtubule seeds / Miho Katsuki, Etsuko Muto, and Robert A. Cross -- Reconstitution and quantification of dynamic microtubule end tracking in vitro using TIRF microscopy / Ivo A. Telley, Peter Bieling, and Thomas Surrey -- Force generation by dynamic microtubules in vitro / Svenja-Marei Kalisch, Liedewij Laan, and Marileen Dogterom -- Variations on the single-molecule assay for microtubule-associated proteins and kinesins / Susanne Bechstedt ... [et al.] -- Analysing the ATP turnover cycle of microtubule motors / Claire T. Friel, Clive R. Bagshaw, and Jonathon Howard -- Cryo-electron tomography of microtubules assembled in vitro from purified components / Frederic M. Coquelle ... [et al.] -- Kinetochore microtubules, analyzed by correlated light and immunoelectron microscopy / Andreas Merdes and Jan De Mey -- Photoactivatable-GFP-a-tubulin as a tool to study microtubule plus-end turnover in living human cells / Catarina P. Samora and Andrew D. McAinsh -- Quantification of asymmetric microtubule nucleation at subcellular structures / Xiaodong Zhu and Irina Kaverina -- Investigating microtubule dynamic instability using microtubule-targeting agents / Stephane Honore and Diane Braguer -- Laser ablation of the microtubule cytoskeleton : setting up and working with an ablation system / Nicola Maghelli and Iva M. Tolic-Norrelykke -- Isolation, identification, and validation of microtubule-associated proteins from Drosophila embryos / Robin Antrobus and James G. Wakefield -- Isolation of novel +TIPs and their binding partners using affinity purification techniques / Ka Lou Yu ... [et al.].
  • 9781617792519
  • 1617792519
  • 9781617792526 (e-isbn)
  • 1617792527 (e-isbn)
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