Western blotting : methods and protocols / edited by Biji T. Kurien, R. Hal Scofield.

Format
Book
Language
English
Published/​Created
New York : Humana Press, [2015]
Description
xiv, 509 pages : illustrations and still photographic images (mostly color) ; 26 cm.

Details

Subject(s)
Western immunoblottingLaboratory manuals [Browse]
Editor
Series
Bibliographic references
Includes bibliographical references and index.
Contents
Early days of blotting / Edwin Southern -- Origins of protein blotting / Harry Towbin -- Western blotting : remembrance of things past / W. Neal Burnette -- Simian virus 40 and protein transfer / Jaime Renart -- Western blotting : an introduction / Biji T. Kurien and R. Hal Scofield -- From little helpers to automation / Biji T. Kurien and Harry Towbin -- Spectrophotometric methods to determine protein concentration / J. P. Dean Goldring -- Solubilization of proteins : the importance of lysis buffer choice / Mandy Peach [and three others] -- Simultaneous immunoblotting analysis with activity gel electrophoresis and 2-D gel electrophoresis / Der-Yen Lee and Geen-Dong Chang -- Diffusion blotting : a rapid and simple method for production of multiple blots from a single gel / Ingrid Olsen and Harald G. Wiker -- Multiple immunoblots by passive diffusion of proteins from a single SDS-PAGE gel / Biji T. Kurien and R. Hal Scofield -- Slice blotting / Graeme Lowe -- Localizing proteins by tissue printing / Rafael F. Pont-Lezica -- Dot-immunobinding assay (Dot-Iba) / Sumi Surendran, Annamma Mathai, and Vishnampet Venkataraman Radhakrishnan -- Analysis of antibody clonotype by affinity immunoblotting / Biji T. Kurien and R. Hal Scofield -- Glycosaminoglycan blotting and detection after electrophoresis separation / Nicola Volpi and Francesca Maccari -- Well-based reverse-phase protein array of formalin- fixed paraffin-embedded tissue / Joon-Yong Chung and Stephen M. Hewitt -- Quantitative computerized western blotting in detail / Dalit Talmi-Frank, Charles L. Jaffe, and Gad Baneth -- Cationic electrophoresis and eastern blotting / Engelbert Buxbaum -- Miniaturized blotting system for simultaneous detection of different autoantibodies / Ulrich Canzler [and eight others] -- Proteomic expressional profiling of a paraffin-embedded tissue by multiplex tissue immunoblotting / Joon-Yong Chungand Stephen M. Hewitt -- Post-staining electroblotting for efficient and reliable peptide blotting / Der-Yen Lee and Geen-Dong Chang -- Multistrip western blotting : a tool for comparative quantitative analysis of multiple proteins / Edita Aksamitiene, Jan B. Hoek, and Anatoly Kiyatkin -- Western blotting using PVDF membranes and its downstream applications / Setsuko Komatsu -- Blotting from phastGel to membranes by ultrasound / Joseph Kost and Aharon Azagury -- Western blotting of high and low molecular weight proteins using heat / Biji T. Kurien and R. Hal Scofield -- Membrane strip affinity purification of autoantibodies / Biji T. Kurien -- Strip immunoblotting of multiple antigenic peptides / Biji T. Kurien -- Double-blotting : a solution to the problem of nonspecific binding of secondary antibodies in immunoblotting procedures / Françoise Lasne -- Method for resolution and western blotting of very large proteins using agarose electrophoresis / Marion L. Greaser and Chad M. Warren -- Immunodetection of P-selectin using an antibody to its C-terminal tag / Padmaja Mehta-D'souza -- Improvements and variants of the multiple antigen blot assay-MABA : an immunoenzymatic technique for simultaneous antigen and antibody screening / Oscar Noya [and six others] -- Blotting from immobilized pH gradient gels : application to total cell lysates / Harry Towbin -- Immunoprecipitation : western blot for proteins of low abundance / Edward P. Trieu and Ira N. Targoff -- Native electrophoresis and western blot analysis (NEWeB) : methods and applications / Ioannis N. Manoussopoulos and Mina Tsagris -- Shift-western blotting : separate analysis of protein and DNA from protein-DNA complexes / Matthias Harbers -- Grid-immunoblotting / Olga Yeliosof and R. Hal Scofield -- Detection and quantification of protein-protein interactions by far-western blotting / Joshua A. Jadwin, Bruce J. Mayer, and Kazuya Machida -- Western blot analysis of adhesive interactions under fluid shear conditions : the blot rolling assay / Robert Sackstein and Robert Fuhlbrigge -- Centrifuge blotting / Jinny Paul -- Blotting of coomassie blue-stained proteins from PAGE gels to transparencies / Jinny Paul -- B-cell ELISPOT : for the identification of antigen-specific antibody-secreting cells / Hemangi B. Shah and Kristi A. Koelsch -- T cell ELISPOT : for the identification of specific cytokine-secreting T cells / Kerry M. Leehan and Kristi A. Koelsch -- Membrane microplates for one- and two-color ELISPOT and FLUOROSPOT assays / Alexander E. Kalyuzhny -- SDS-PAGE to immunoblot in one hour / Biji T. Kurien [and three others] -- Single-cell western blotting / Syed M. S. Quadri -- Protein detection by simple western analysis / Valerie M. Harris -- Western blotting using microfluidics / Pothur R. Srinivas -- Two-dimensional gel-based protein standardization verified by western blot analysis / Hisao Haniu [and three others] -- Fingerprint deposition on nitrocellulose and polyvinylidene difluoride membranes using alkaline phosphatase / Biji T. Kurien, Debashish Danda, and R. Hal Scofield -- Other notable protein blotting methods : a brief review / Biji T. Kurien and R. Hal Scofield.
Other format(s)
Also available in an electronic version.
ISBN
  • 9781493926930 (alk. paper)
  • 1493926934 (alk. paper)
LCCN
2015938704
OCLC
904811969
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