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Antibody engineering [electronic resource] : methods and protocols / edited by Benny K.C. Lo.
Format
Book
Language
English
Published/Created
Totowa, N.J. : Humana Press, c2004.
Description
xiv, 561 p. : ill. ; 24 cm.
Availability
Available Online
Online Content
Online Content
SpringerNature Complete Protocols
Details
Subject(s)
Immunotechnology
—
Laboratory manuals
[Browse]
Related name
Lo, Benny K. C.
[Browse]
SpringerLink (Online service)
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Library of Congress genre(s)
Laboratory manuals
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Series
Methods in molecular biology (Clifton, N.J.) ; v. 248.
[More in this series]
Methods in molecular biology ; 248
Bibliographic references
Includes bibliographical references and index.
System details
System requirements: World Wide Web browser, internet connectivity, Adobe Acrobat reader.
Mode of access: World Wide Web.
Contents
Cover
Preface
Contents
Contributors
I ANTIBODY SEQUENCE AND STRUCTURE
1 Internet Resources for the Antibody Engineer
2 The Kabat Database and a Bioinformatics Example
3 IMGT, The International ImMunoGeneTics Information System®, http://imgt.cines.fr
4 Antibody Variable Regions: Toward a Unified Modeling Method
5 Studying Antibody Conformations by Ultracentrifugation and Hydrodynamic Modeling
II ANTIBODY-LEAD GENERATION
6 PCR Cloning of Human Immunoglobulin Genes
7 Antibody Humanization by CDR Grafting
8 Selection of Human Antibodies from Phage Display Libraries
9 Production of Human Single-Chain Antibodies by Ribosome Display
10 Production of Human Antibodies from Transgenic Mice
11 Selection of Internalizing Antibodies for Drug Delivery
12 Engineering Multivalent Antibody Fragments for In Vivo Targeting
13 Production of Recombinant Bispecific Antibodies
III ANTIBODY EXPRESSION AND OPTIMIZATION
14 Expression and Isolation of Recombinant Antibody Fragments in E. coli
15 Expression of Recombinant Antibodies in Mammalian Cell Lines
16 Human Antibody Production Using Insect-Cell Expression Systems
17 Antibody Production in Transgenic Plants
18 Directed Mutagenesis of Antibody Variable Domains
19 Antibody Affinity Maturation by Chain Shuffling
20 Antibody Affinity Maturation by Random Mutagenesis
21 Developing a Minimally Immunogenic Humanized Antibody by SDR Grafting
IV ANTIBODY CHARACTERIZATION AND NOVEL APPLICATIONS
22 Antibody Purification by Column Chromatography
23 Affinity Measurement Using Surface Plasmon Resonance
24 Kinetic Exclusion Assays to Study High-Affinity Binding Interactions in Homogeneous Solutions
25 Characterization of Antibody ... Antigen Interactions by Fluorescence Spectroscopy
26 Antibody Epitope Mapping Using Arrays of Synthetic Peptides
27 Mapping Antibody:Antigen Interactions by Mass Spectrometry and Bioinformatics
28 Radiometal Labeling of Antibodies and Antibody Fragments for Imaging and Therapy
29 Production and Characterization of Anti-Cocaine Catalytic Antibodies
30 Recombinant Immunotoxins in the Treatment of Cancer
31 Antibodies in Proteomics
32 Targeting of Antibodies Using Aptamers.
Show 37 more Contents items
Other format(s)
Also available on the Internet to registered users.
ISBN
1588290921 (alk. paper)
9781588290922 (alk. paper)
9781592596669 (electronic bk.)
1592596665 (electronic bk.)
6610358389 (electronic bk.)
9786610358380 (electronic bk.)
OCLC
170925992
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Other versions
Antibody engineering : methods and protocols / edited by Benny K.C. Lo.
id
9940933203506421
Antibody Engineering [electronic resource] : Methods and Protocols / edited by Benny K. C. Lo.
id
99125262256206421