Antibody engineering [electronic resource] : methods and protocols / edited by Benny K.C. Lo.

Format
Book
Language
English
Published/​Created
Totowa, N.J. : Humana Press, c2004.
Description
xiv, 561 p. : ill. ; 24 cm.

Details

Subject(s)
Library of Congress genre(s)
Series
  • Methods in molecular biology (Clifton, N.J.) ; v. 248. [More in this series]
  • Methods in molecular biology ; 248
Bibliographic references
Includes bibliographical references and index.
System details
  • System requirements: World Wide Web browser, internet connectivity, Adobe Acrobat reader.
  • Mode of access: World Wide Web.
Contents
  • Cover
  • Preface
  • Contents
  • Contributors
  • I ANTIBODY SEQUENCE AND STRUCTURE
  • 1 Internet Resources for the Antibody Engineer
  • 2 The Kabat Database and a Bioinformatics Example
  • 3 IMGT, The International ImMunoGeneTics Information System®, http://imgt.cines.fr
  • 4 Antibody Variable Regions: Toward a Unified Modeling Method
  • 5 Studying Antibody Conformations by Ultracentrifugation and Hydrodynamic Modeling
  • II ANTIBODY-LEAD GENERATION
  • 6 PCR Cloning of Human Immunoglobulin Genes
  • 7 Antibody Humanization by CDR Grafting
  • 8 Selection of Human Antibodies from Phage Display Libraries
  • 9 Production of Human Single-Chain Antibodies by Ribosome Display
  • 10 Production of Human Antibodies from Transgenic Mice
  • 11 Selection of Internalizing Antibodies for Drug Delivery
  • 12 Engineering Multivalent Antibody Fragments for In Vivo Targeting
  • 13 Production of Recombinant Bispecific Antibodies
  • III ANTIBODY EXPRESSION AND OPTIMIZATION
  • 14 Expression and Isolation of Recombinant Antibody Fragments in E. coli
  • 15 Expression of Recombinant Antibodies in Mammalian Cell Lines
  • 16 Human Antibody Production Using Insect-Cell Expression Systems
  • 17 Antibody Production in Transgenic Plants
  • 18 Directed Mutagenesis of Antibody Variable Domains
  • 19 Antibody Affinity Maturation by Chain Shuffling
  • 20 Antibody Affinity Maturation by Random Mutagenesis
  • 21 Developing a Minimally Immunogenic Humanized Antibody by SDR Grafting
  • IV ANTIBODY CHARACTERIZATION AND NOVEL APPLICATIONS
  • 22 Antibody Purification by Column Chromatography
  • 23 Affinity Measurement Using Surface Plasmon Resonance
  • 24 Kinetic Exclusion Assays to Study High-Affinity Binding Interactions in Homogeneous Solutions
  • 25 Characterization of Antibody ... Antigen Interactions by Fluorescence Spectroscopy
  • 26 Antibody Epitope Mapping Using Arrays of Synthetic Peptides
  • 27 Mapping Antibody:Antigen Interactions by Mass Spectrometry and Bioinformatics
  • 28 Radiometal Labeling of Antibodies and Antibody Fragments for Imaging and Therapy
  • 29 Production and Characterization of Anti-Cocaine Catalytic Antibodies
  • 30 Recombinant Immunotoxins in the Treatment of Cancer
  • 31 Antibodies in Proteomics
  • 32 Targeting of Antibodies Using Aptamers.
Other format(s)
Also available on the Internet to registered users.
ISBN
  • 1588290921 (alk. paper)
  • 9781588290922 (alk. paper)
  • 9781592596669 (electronic bk.)
  • 1592596665 (electronic bk.)
  • 6610358389 (electronic bk.)
  • 9786610358380 (electronic bk.)
OCLC
170925992
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