T cell protocols / edited by Gennaro De Libero.

Format
Book
Language
English
Εdition
2nd ed.
Published/​Created
New York : Humana Press, ©2009.
Description
xi, 165 pages, 3 unnumbered pages of plates : illustrations

Availability

Copies in the Library

Location Call Number Status Location Service Notes
Lewis Library - Lewis Library - Serials (Off-Site) QH506 .M45 1984 vol.514 Browse related items Request

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    Summary note
    • With a wide variety of investigative approaches. T cell immunology is a vital and open field of study. In T Cell Protocols, Second Edition, an international panel of experts contribute fully updated classic protocols as well as newly established novel techniques for the study of T lymphocyte biology. Written in the highly successful Methods in Molecular BiologyTM series format, the chapters in this volume provide brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections which collect expert tips on troubleshooting and avoiding known pitfalls.
    • Up-to-date and easy to use, T Cell Protocols, Second Edition is an ideal guide for young investigators new to the complex field of immunology as well as a valuable, concise resource for experienced scientists searching for clear, efficacious descriptions of novel methods.
    Bibliographic references
    Includes bibliographical references and index.
    Contents
    • 1. Analysis of frequency and phenotype of antigen-specific T cells / Angus Stock and Vincenzo Cerundolo
    • 2. B cell helper assays / Sergio Abrignani, ... [et al.]
    • 3. transkingdom RNA interference (tkRNAi) / Thu A. Nguyen and Johannes H. Fruehauf
    • 4. Flow cytometry and cell activation / Sonia Gavasso
    • 5. Investigating T cells by polychromatic flow cytometry / Enrico Lugli, Leonarda Troiano, and Andrea Cossarizza
    • 6. Generation of human T cell clones / Sabrina Mariotti and Roberto Nisini
    • 7. Limiting dilution analysis of antigen-specific T cells / Jorge Carneiro, Lurdes Duarte, and Elisabetta Padovan
    • 8. T cell epitope-mapping by cytokine gene expression assay / Maurizio Provengano and Giulio C. Spagnoli
    • 9. Cytokine multiplex immunoassay / Wilco de Jager, Berent Prakken, and Ger T. Rijkers
    • 10. Purification of the T cell antigen receptor and analysis by blue-native PAGE / Mahima Swamy and Wolfgang W.A. Schamel
    • 11. Non-replicating recombinant vaccinia virus expressing CD80 to enhance T-cell stimulation / Paul Zajac.
    Other format(s)
    Also available in an electronic version.
    ISBN
    • 9781588295873 ((hbk. ; : alk. paper))
    • 1588295877 ((hbk. ; : alk. paper))
    • 9781603275279 ((e-ISBN))
    • 1603275274 ((e-ISBN))
    LCCN
    2008941671
    OCLC
    227033896
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