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T cell protocols / edited by Gennaro De Libero.
Format
Book
Language
English
Εdition
2nd ed.
Published/Created
New York : Humana Press, ©2009.
Description
xi, 165 pages, 3 unnumbered pages of plates : illustrations
Availability
Available Online
Online Content
SpringerNature Complete Protocols
Copies in the Library
Location
Call Number
Status
Location Service
Notes
Lewis Library - Lewis Library - Serials (Off-Site)
QH506 .M45 1984 vol.514
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Details
Subject(s)
T cells
—
Laboratory manuals
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Related name
De Libero, Gennaro
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Library of Congress genre(s)
Laboratory manuals
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Series
Methods in molecular biology (Clifton, N.J.) ; v. 514.
[More in this series]
Springer protocols (Series)
[More in this series]
Methods in molecular biology, 1064-3745 ; 514
Springer protocols
Summary note
With a wide variety of investigative approaches. T cell immunology is a vital and open field of study. In T Cell Protocols, Second Edition, an international panel of experts contribute fully updated classic protocols as well as newly established novel techniques for the study of T lymphocyte biology. Written in the highly successful Methods in Molecular BiologyTM series format, the chapters in this volume provide brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections which collect expert tips on troubleshooting and avoiding known pitfalls.
Up-to-date and easy to use, T Cell Protocols, Second Edition is an ideal guide for young investigators new to the complex field of immunology as well as a valuable, concise resource for experienced scientists searching for clear, efficacious descriptions of novel methods.
Bibliographic references
Includes bibliographical references and index.
Contents
1. Analysis of frequency and phenotype of antigen-specific T cells / Angus Stock and Vincenzo Cerundolo
2. B cell helper assays / Sergio Abrignani, ... [et al.]
3. transkingdom RNA interference (tkRNAi) / Thu A. Nguyen and Johannes H. Fruehauf
4. Flow cytometry and cell activation / Sonia Gavasso
5. Investigating T cells by polychromatic flow cytometry / Enrico Lugli, Leonarda Troiano, and Andrea Cossarizza
6. Generation of human T cell clones / Sabrina Mariotti and Roberto Nisini
7. Limiting dilution analysis of antigen-specific T cells / Jorge Carneiro, Lurdes Duarte, and Elisabetta Padovan
8. T cell epitope-mapping by cytokine gene expression assay / Maurizio Provengano and Giulio C. Spagnoli
9. Cytokine multiplex immunoassay / Wilco de Jager, Berent Prakken, and Ger T. Rijkers
10. Purification of the T cell antigen receptor and analysis by blue-native PAGE / Mahima Swamy and Wolfgang W.A. Schamel
11. Non-replicating recombinant vaccinia virus expressing CD80 to enhance T-cell stimulation / Paul Zajac.
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Other format(s)
Also available in an electronic version.
ISBN
9781588295873 ((hbk. ; : alk. paper))
1588295877 ((hbk. ; : alk. paper))
9781603275279 ((e-ISBN))
1603275274 ((e-ISBN))
LCCN
2008941671
OCLC
227033896
Statement on language in description
Princeton University Library aims to describe library materials in a manner that is respectful to the individuals and communities who create, use, and are represented in the collections we manage.
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Other versions
T Cell Protocols [electronic resource] / edited by Gennaro De Libero.
id
99125163561206421
T cell protocols / edited by Gennaro De Libero.
id
SCSB-5446829