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Cellular electron microscopy / edited by J. Richard McIntosh.
Format
Book
Language
English
Published/Created
Amsterdam ; Boston : Elsevier/Academic Press, ©2007.
Description
xxvii, 850 pages, 28 unnumbered pages of plates : illustrations.
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Status
Location Service
Notes
ReCAP - Remote Storage
QH585 .M47 vol.79
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Details
Subject(s)
Electron microscopy
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Microscopy
—
Technique
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Cell physiology
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Related name
McIntosh, J. Richard
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Series
Methods in cell biology ; v. 79.
[More in this series]
Methods in cell biology, 0091-679X ; v. 79
[More in this series]
Summary note
"Recent improvements in electron microscopy of cells are bringing cell biologists back to "EM", particularly as they realize the limitations of the light microscopic methods that have been dominating the study of cell structure. This trend is a direct result of the resolving power of the electron microscope, of improved methods for specimen preparation, and of new technologies for three-dimensional imaging and image analysis. EM is beginning to fulfill its promise of linking light microscopy of live cells with atomic resolution studies of isolated macromolecules, with the goal of improving our understanding of living systems at atomic resolution. These new methods will help biologists from many disciplines to apply modern EM to their own work, allowing them to participate in the groundbreaking discoveries that are now being made in this field." "Cellular Electron Microscopy is divided into five sections, each beginning with an introduction by the editor. The sections cover many issues: specimen preparation, 3D imaging, use of frozen-hydrated samples, labeling macromolecules, and technologies for data aquisition and analysis. This book is explicitly designed for new and potential users, not experts in EM, so the chapters focus on practical information, including discussions of why each topic is important and its likely future developments. The work will prove an invaluable resource for anyone in cell biology, as well as those in biophysics, microbiology, neuroscience, microscopy, biochemistry, and genetics. Book jacket."--BOOK JACKET.
Bibliographic references
Includes bibliographical references and index.
Contents
The Physics of Rapid Cooling and Its Implications for Cryoimmobilization of Cells / J. Dubochet
Cryopreparation Methods for Electron Microscopy of Selected Model Systems / K. McDonald
Cryopreparation Methodology for Plant Cell Biology / M.W. Hess
Correlative Light and Electron Microscopy of Early Caenorhabditis elegans Embryos in Mitosis / T. Muller-Reichert
Understanding Microtubule Organizing Centers by Comparing Mutant and Wild-Type Structures with Electron Tomography / E.T. O'Toole
Whole-Cell Investigation of Microtubule Cytoskeleton Architecture by Electron Tomography / J.L. Hoog
Electron Microscopy of Archaea / C.E. Robertson
Reconstructing Mammalian Membrane Architecture by Large Area Cellular Tomography / B.J. Marsh
Visualization of Membrane-Cytoskeletal Interactions During Plant Cytokinesis / M.S. Otegui
Electron Tomographic Methods for Studying the Chemical Synapse / C.P. Arthur
Using Electron Microscopy to Understand Functional Mechanisms of Chromosome Alignment on the Mitotic Spindle / B.F. McEwen
Electron Microscopic Analysis of the Leading Edge in Migrating Cells / T. Svitkina
Imaging Actomyosin In Situ / K.A. Taylor
Electron Tomography of Bacterial Chemotaxis Receptor Assemblies / P. Zhang
How to "Read" a Vitreous Section / J. Dubochet
Single-Particle Electron Cryomicroscopy of the Ion Channels in the Excitation-Contraction Coupling Junction / I.I. Serysheva
Electron Microscopy of Microtubule-Based Cytoskeletal Machinery / A. Hoenger
Reconstructing the Endocytotic Machinery / Y. Cheng
3D Immunolocalization with Plastic Sections / M.K. Morphew
Electron Microscopy Analysis of Viral Morphogenesis / A. Pelchen-Matthews
Electron Tomography of Immunolabeled Cryosections / M.S. Ladinsky
Visualizing Macromolecules with Fluoronanogold: From Photon Microscopy to Electron Tomography / T. Cheutin
Markers for Correlated Light and Electron Microscopy / G.E. Sosinsky
Localizing Specific Elements Bound to Macromolecules by EFTEM / R.D. Leapman
Localization of Protein Complexes by Pattern Recognition / C. Best
The Application of Energy-Filtered Electron Microscopy to Tomography of Thick, Selectively Stained Biological Samples / J.C. Bouwer
Optimization of Image Collection for Cellular Electron Microscopy / P. Mooney
Future Directions for Camera Systems in Electron Microscopy / N. Xuong
Structure Determination In Situ by Averaging of Tomograms / F. Forster
Methods for Image Segmentation in Cellular Tomography / K. Sandberg
Database Resources for Cellular Electron Microscopy / M.E. Martone.
Show 28 more Contents items
ISBN
9780123706478 ((alk. paper))
0123706475 ((alk. paper))
LCCN
2008357277
OCLC
85847813
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Cellular electron microscopy / edited by J. Richard McIntosh.
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