Cellular electron microscopy / edited by J. Richard McIntosh.

Format
Book
Language
English
Published/​Created
Amsterdam ; Boston : Elsevier/Academic Press, ©2007.
Description
xxvii, 850 pages, 28 unnumbered pages of plates : illustrations.

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    Summary note
    "Recent improvements in electron microscopy of cells are bringing cell biologists back to "EM", particularly as they realize the limitations of the light microscopic methods that have been dominating the study of cell structure. This trend is a direct result of the resolving power of the electron microscope, of improved methods for specimen preparation, and of new technologies for three-dimensional imaging and image analysis. EM is beginning to fulfill its promise of linking light microscopy of live cells with atomic resolution studies of isolated macromolecules, with the goal of improving our understanding of living systems at atomic resolution. These new methods will help biologists from many disciplines to apply modern EM to their own work, allowing them to participate in the groundbreaking discoveries that are now being made in this field." "Cellular Electron Microscopy is divided into five sections, each beginning with an introduction by the editor. The sections cover many issues: specimen preparation, 3D imaging, use of frozen-hydrated samples, labeling macromolecules, and technologies for data aquisition and analysis. This book is explicitly designed for new and potential users, not experts in EM, so the chapters focus on practical information, including discussions of why each topic is important and its likely future developments. The work will prove an invaluable resource for anyone in cell biology, as well as those in biophysics, microbiology, neuroscience, microscopy, biochemistry, and genetics. Book jacket."--BOOK JACKET.
    Bibliographic references
    Includes bibliographical references and index.
    Contents
    • The Physics of Rapid Cooling and Its Implications for Cryoimmobilization of Cells / J. Dubochet
    • Cryopreparation Methods for Electron Microscopy of Selected Model Systems / K. McDonald
    • Cryopreparation Methodology for Plant Cell Biology / M.W. Hess
    • Correlative Light and Electron Microscopy of Early Caenorhabditis elegans Embryos in Mitosis / T. Muller-Reichert
    • Understanding Microtubule Organizing Centers by Comparing Mutant and Wild-Type Structures with Electron Tomography / E.T. O'Toole
    • Whole-Cell Investigation of Microtubule Cytoskeleton Architecture by Electron Tomography / J.L. Hoog
    • Electron Microscopy of Archaea / C.E. Robertson
    • Reconstructing Mammalian Membrane Architecture by Large Area Cellular Tomography / B.J. Marsh
    • Visualization of Membrane-Cytoskeletal Interactions During Plant Cytokinesis / M.S. Otegui
    • Electron Tomographic Methods for Studying the Chemical Synapse / C.P. Arthur
    • Using Electron Microscopy to Understand Functional Mechanisms of Chromosome Alignment on the Mitotic Spindle / B.F. McEwen
    • Electron Microscopic Analysis of the Leading Edge in Migrating Cells / T. Svitkina
    • Imaging Actomyosin In Situ / K.A. Taylor
    • Electron Tomography of Bacterial Chemotaxis Receptor Assemblies / P. Zhang
    • How to "Read" a Vitreous Section / J. Dubochet
    • Single-Particle Electron Cryomicroscopy of the Ion Channels in the Excitation-Contraction Coupling Junction / I.I. Serysheva
    • Electron Microscopy of Microtubule-Based Cytoskeletal Machinery / A. Hoenger
    • Reconstructing the Endocytotic Machinery / Y. Cheng
    • 3D Immunolocalization with Plastic Sections / M.K. Morphew
    • Electron Microscopy Analysis of Viral Morphogenesis / A. Pelchen-Matthews
    • Electron Tomography of Immunolabeled Cryosections / M.S. Ladinsky
    • Visualizing Macromolecules with Fluoronanogold: From Photon Microscopy to Electron Tomography / T. Cheutin
    • Markers for Correlated Light and Electron Microscopy / G.E. Sosinsky
    • Localizing Specific Elements Bound to Macromolecules by EFTEM / R.D. Leapman
    • Localization of Protein Complexes by Pattern Recognition / C. Best
    • The Application of Energy-Filtered Electron Microscopy to Tomography of Thick, Selectively Stained Biological Samples / J.C. Bouwer
    • Optimization of Image Collection for Cellular Electron Microscopy / P. Mooney
    • Future Directions for Camera Systems in Electron Microscopy / N. Xuong
    • Structure Determination In Situ by Averaging of Tomograms / F. Forster
    • Methods for Image Segmentation in Cellular Tomography / K. Sandberg
    • Database Resources for Cellular Electron Microscopy / M.E. Martone.
    ISBN
    • 9780123706478 ((alk. paper))
    • 0123706475 ((alk. paper))
    LCCN
    2008357277
    OCLC
    85847813
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