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Princeton University Library Catalog
Advanced methods in structural biology / edited by Ângela Sousa and Luis Passarinha.
New York, NY : Humana Press, 
xv, 467 pages : illustrations (some color) ; 26 cm.
Sousa, Ângela (Ângela Maria Almeida de)
Methods in molecular biology (Clifton, N.J.) ; v. 2652.
[More in this series]
Springer protocols (Series)
[More in this series]
Methods in molecular biology, 1064-3745 ; 2652
This volume explores the latest integrated bioprocesses and technologies used to study the production of the target recombinant protein of therapeutic or diagnostic interest; its isolation, purification, and stabilization; and the bio-interaction and structural analyses. The chapters in this book are organized into four parts. Part One covers production methods of soluble and membrane proteins in prokaryotic and eukaryotic expression systems, such as Lactococcus lactis and Escherichia coli. Part Two describes traditional and novel approaches for recombinant protein purification and stabilization, and buffers and additives. Part Three discusses automated methods in structural biology based on in silico approaches; and Part Four provides examples of advanced protein investigation methodologies to assess structural analysis such as high-throughput protein crystallization and time-resolved serial crystallography. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Advanced Methods in Structural Biology is a valuable resource to those in academia (i.e., graduate students and postdoctoral researchers) and researchers in the pharmaceutical industry who wish to learn more about this developing field. Chapter 5 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Includes bibliographical references and index.
Rights and reproductions note
Current copyright fee: GBP19.00 42\0.
Cloning and Recombinant Protein Expression in Lactococcus lactis
E6 Tagged Protein Production, Extraction, and Purification from Escherichia coli Lysate
Mini-Bioreactor Platform for Membrane Proteins Production in Komagataella pastoris
Recombinant Protein Production Using the Baculovirus Expression Vector System (BEVS)
High-Throughput Production and Optimization of Membrane Proteins after Expression in Mammalian Cells
Fluorescent Fusion Proteins Expression in Plant Cells
Functional Expression of the Ectodomain of Plant Receptor Kinases in Plant Suspension Culture
A Simple Purification Method for Heat Stable Recombinant Low Molecular Weight Proteins and Peptides Via GST-Fusion Products
eGFP as an All-in-One Tag for Purification of Membrane Proteins
Effects of Ionic Liquids as Additives on Protein Crystallization
Screening of Buffers and Additives for Protein Stabilization by Thermal Shift Assay: A Practical Approach
Biophysical Characterization of Membrane Proteins
Expression in Saccharomyces cerevisiae and Purification of a Human Phospholipid Flippase
Preparation of Protein-Enriched Outer Membrane Vesicles from Escherichia Coli for In Situ Structural Biology of Outer Membrane Proteins
Receptor-Based Virtual Screening of Large Libraries in a Multi-Level in Silico Approach
Protein-Protein Interaction Studies Using Molecular Dynamics Simulation
Isolation of Bacterial Extracellular Vesicles and Identification of their Protein Cargo
Study of Protein Conformational Dynamics Using Hydrogen/Deuterium Exchange Mass Spectrometry
Protein Interaction Analysis by Surface Plasmon Resonance
High-Throughput Protein Crystallization in an Integrated Droplet-Based Microfluidic Platform
Sample Preparation for Time-Resolved Serial Crystallography: Practical Considerations
Using Small-Angle X-Ray Scattering to Characterize Biological Systems: A General Overview and Practical Tips
Nuclear Magnetic Resonance-Guided Structural Analysis of Moderate-Affinity Protein Complexes with Intrinsically Disordered Polypeptides
Protein Structural Analysis by Cryogenic Electron Microscopy.
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Advanced Methods in Structural Biology