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Princeton University Library Catalog
Chromatin accessibility : methods and protocols / edited by Georgi K. Marinov and William J. Greenleaf.
New York, NY : Humana Press, 
xi, 341 pages : illustrations (some color) ; 26 cm.
Marinov, Georgi K.
Greenleaf, William James
Methods in molecular biology (Clifton, N.J.) ; v. 2611.
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Springer protocols (Series)
[More in this series]
Methods in molecular biology, 1064-3745 ; 2611
This detailed volume provides a comprehensive resource covering the existing and state-of-the-art tools in the field of profiling chromatin accessibility and its dynamics. Beginning with a section on bulk-cell methods for profiling chromatin accessibility and nucleosome positioning that rely on enzymatic cleavage of accessible DNA and produce information about relative accessibility, the book continues with methods that use single-molecule and enzymatic approaches to solving the problem of mapping absolute occupancy/accessibility, emerging tools for mapping DNA accessibility and nucleosome positioning in single cells, imaging-based methods for visualizing accessible chromatin in its nuclear context, as well as computational methods for the processing and analysis of chromatin accessibility datasets. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Chromatin Accessibility: Methods and Protocols serves as an extensive and useful reference for researchers studying different facets of chromatin accessibility in a wide variety of biological contexts. Chapter 6 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Includes bibliographical references and index.
Genome-Wide Mapping of Active Regulatory Elements Using ATAC-Seq
Mapping Nucleosome Location Using FS-Seq
NicE-Seq: A Simple and Quick Method for Accessible Chromatin Detection in Fixed Cells
Measuring Inaccessible Chromatin Genome-Wide Using Protect-Seq
Determination of the Chromatin Openness in Bacterial Genomes
Profiling Chromatin Accessibility on Replicated DNA with Repli-ATAC-Seq
Analysis of Chromatin Interaction and Accessibility by Trac-Looping
Single-Molecule Mapping of Chromatin Accessiility Using NOMe-Seq/dSMF
ORE-Seq: Genome-Wide Absolute Occupancy Measurement by Restriction Enzyme Accessibilities
Single-Cell Joint Profiling of Open Chromatin and Transcriptome by Paired-Seq
Simultaneous Single-Cell Profiling of the Transcriptome and Accessible Chromatin Using SHARE-Seq
Simultaneous Measurement of DNA Methylation and Nucleosome Occupancy in Single Cells Using scNOMe-Seq
Massively Parallel Profiling of Accessible Chromatin and Proteins with ASAP-Seq
Concomitant Sequencing of Accessible Chromatin and Mitochondrial Genomes in Single Cells Using mtscATAC-Seq
ATAC-See: A Tn5 Transposase-Mediated Assay for Detection of Chromatin Accessibility with Imaging
NicE-viewSeq: An Integrative Visualization and Genomics Method to Detect Accessible Chromatin in Fixed Cells
ATAC-Seq Data Processing
Deep Learning on Chromatin Accessibility.
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