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Princeton University Library Catalog
Cartilage tissue engineering / edited by Martin J. Stoddart and Elena Della Bella, Angela R. Armiento.
New York, NY : Humana Press, 
xv, 393 pages : illustrations (some color) ; 26 cm.
Stoddart, Martin J.
Della Bella, Elena
Armiento, Angela R.
Methods in molecular biology (Clifton, N.J.) ; v. 2598.
[More in this series]
Springer protocols (Series)
[More in this series]
Methods in molecular biology, 1064-3745 ; 2598
This detailed book brings together a collection of methodologies, from the most basic to the more complex, that provides researchers with a platform they can use to embark on a cartilage research career. To aid in the search for novel therapies for cartilage regeneration, this volume addresses 3D cartilage models, challenges associated with RNA and protein extraction, imaging, gene transfer, as well as stable differentiation and variations in cell phenotype from different tissue origins. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Cartilage Tissue Engineering serves as an ideal guide for researchers working to advance the vital study of cartilage biology and repair.
Includes bibliographical references and index.
Rights and reproductions note
Current copyright fee: GBP19.00 42\0.
Cartilage Tissue Engineering: An Introduction
Chondrocyte Isolation and Expansion
Isolation of Chondrons from Hyaline Cartilage
Articular Cartilage Chondroprogenitors: Isolation and Directed Differentiation
Physioxic Culture of Chondrogenic Cells
Isolation and In Vitro Chondrogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stromal Cells
Adipose-Derived Stromal Cells: Isolation, Expansion, and Differentiation
Chondrogenic Differentiation of Human Induced Pluripotent Stem Cells
The Dimethylmethylene Blue Assay (DMMB) for the Quantification of Sulphated Glycosaminoglycans
Extracellular Vesicle Isolation and Characterization for Applications in Cartilage Tissue Engineering and Osteoarthritis Therapy
Metabolomic Profiling to Understand Chondrocyte Metabolism
Techniques for Visualization and Quantification of Primary Cilia in Chondrocytes
Co-Culture of Human Articular Chondrocytes Seeded in Polyurethane Scaffolds and Human Mesenchymal Stromal Cells Encapsulated in Alginate Beads
Isolation of Murine Articular Chondrocytes for Single Cell RNA or Bulk RNA Sequencing Analysis
Modulation of MicroRNA Expression during In Vitro Chondrogenesis
Purification and Isolation of Proteins from Hyaline Cartilage
Histological Preparation and Evaluation of Cartilage Specimens
Articular Cartilage Imaging in the Context of the Superficial Chondrocyte Spatial Organization (SCSO) as a Surrogate Marker for Functional Pathology
Unconfined Compression Experimental Protocol for Cartilage Explants and Hydrogel Constructs: From Sample Preparation to Mechanical Characterization
Viral Gene Delivery in Chondrocytes
Decellularization of Articular Cartilage: A Hydrochloric Acid-Based Strategy
Self-Assembly Culture Model for Engineering Musculoskeletal Tissues
Osteochondral Explants Isolation and Culture under a Compression and Shear Bioreactor
A Mouse Model of Acute Cartilage Injury and Repair
Small-Animal Compression Models of Osteoarthritis
Modelling and Assessing Osteoarthritis in Mice by Destabilization of the Medial Meniscus (DMM)
Collagenase-Induced Osteoarthritis in Mice
Platelet-Rich Plasma for Intra-Articular Injections: Preclinical and Clinical Evidence.
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Cartilage Tissue Engineering [electronic resource] / edited by Martin J. Stoddart, Elena Della Bella, Angela R. Armiento.