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Princeton University Library Catalog
CCN proteins : methods and protocols / edited by Masaharu Takigawa.
New York, NY : Humana Press, 
xiv, 430 pages : illustrations (some color) ; 26 cm.
Methods in molecular biology (Clifton, N.J.) ; v. 2582.
[More in this series]
Springer protocols (Series)
[More in this series]
Methods in molecular biology, 1064-3745 ; 2582
This second edition volume expands on the previous edition with updated methodologies and practical tips to overcome obstacles associated with experimentation pertaining to chemistry, biology, physiology, pathology, medical and dental sciences, and pharmacology of CCN proteins. The chapters in this book cover topics such as CCN4 immunofluorescence for tissue microarray; utilizing public molecular biological databases for CCN family research; the effects of CCN4 on pancreatic beta cell proliferation; gene expression analysis of CCNs; novel cell biological assays for measuring bone remodeling activities of CCN proteins; and the function of CCN2 in tubular epithelium cells with a focus on renal fibrogenesis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, CCN Proteins: Methods and Protocols, Second Edition is a valuable resource for experienced CCN researchers looking for new approaches and novice researchers just starting out in the field of CCN research.
Includes bibliographical references and index.
Rights and reproductions note
Current copyright fee: GBP19.00 42\0.
CCN Proteins - Cellular Communication Network Factors: Expanding their Repertoire toward a New Concept
Optimal CCN4 Immunofluorescence for Tissue Microarray
Cellular Fluorescence Imaging for the Evaluation of Bioactivity of CCN Family Proteins- Imaging of Molecular Interaction between CCN Protein and Its Binding Partners: An In Situ Proximity Ligation Assay of Interaction Between CCN2 and Rab14 in Chondrocytes
Western Blot Protocols for Analysis of CCN Proteins and Fragments in Exosomes, Vesicle-free Fractions, and Cells
Comprehensive Method for Exosome Isolation and Proteome Analysis for Detection of CCN Factors in/on Exosomes
Evaluation of the Molecular Interaction between CCN Protein and Its Binding Partners: A Solid-Phase Binding Assay and Surface Plasmon Resonance
Protocols for Screening Peptides Binding to CCN Family Proteins and their Extended Utility
Transfection, Spinfection, Exofection, and Luciferase Assays for Analysis of CCN Genes Expression Mechanism
Analyses of the Post-Transcriptional Regulation of CCN Genes: Approach to Multiple Steps of CCN2 Gene Expression
Protocol for CRISPR/Cas Genome Editing for Investigating Cell Communication Network
Utilizing Public Molecular Biological Databases for CCN Family Research
Evaluation of the Effects of CCN4 on Pancreatic Beta Cell Proliferation
Analysis of CCN Functions in Liver Regeneration After Partial Hepatectomy
The Evaluation of Meniscus Regenerative Effects of LIPUS-Induced CCN Proteins: Induction by LIPUS of CCN2 and Meniscus-Related Genes in Cultured Meniscus Cells and Meniscus Tissues
Analysis of Chemotactic Property of CCN2/CTGF in Intramembranous Osteogenesis
Novel Cell Biological Assays for Measuring Bone Remodeling Activities of CCN Proteins
Imaging Analysis of Neurotrophic Effects by CCN2 Protein in Neuronal Precursor Cells Derived from Human Induced Pluripotent Stem Cells
Gene Expression Analysis of CCNs Along with Odontoblastic Differentiation In Vivo
Angiogenesis Assays for the Analysis of CCN Proteins
Using the Bleomycin-Induced Model of Fibrosis to Study the Contribution of CCN Proteins to Scleroderma Fibrosis
Atomic Force Microscopy-Based Measurements of Retinal Microvessel Stiffness in Mice with Endothelial-Specific Deletion of CCN1
Immunohistochemical Analysis of CCN2 in Experimental Fracture Healing Models
Mouse Models of Tumor Bone Metastasis and Invasion for Studying CCN Proteins
Regulation of CCN2 and Its Bioactivity by Advanced Glycation End Products
Analysis of CCN4/WISP1 Effects on Joint Tissues Using Gain and Loss of Function Approaches
Generation of Conditional KO Mice of CCN2 and Its Function in the Kidney
Analysis of the Function of CCN2 in Tubular Epithelium Cells with a Focus on Renal Fibrogenesis.
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CCN Proteins [electronic resource] : Methods and Protocols / edited by Masaharu Takigawa.