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Princeton University Library Catalog
Bacterial Amyloids [electronic resource] : Methods and Protocols / edited by Véronique Arluison, Frank Wien, Andrés Marcoleta.
1st ed. 2022.
New York, NY : Springer US : Imprint: Humana, 2022.
1 online resource (XI, 337 p. 108 illus., 98 illus. in color.)
Methods in Molecular Biology, 2538
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Methods in Molecular Biology, 1940-6029 ; 2538
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This detailed volume introduces different methods to assess the structure and folding of bacterial amyloids and analyze their functions, either in vitro or in vivo. Despite their initial association with neurodegenerative diseases, there is now increasing evidence of alternative roles for amyloids, with beneficial aspects for cells. In particular, prokaryotes use amyloids as functional assemblies, where they are key players in the cell's physiology. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Bacterial Amyloids: Methods and Protocols is an ideal guide for experts and novices alike to further explore these crucial protein assemblies.
Study of Amyloid Fibers Using Atomic Force Microscopy
Cryo-Electron Microscopy to Analyze the Structure of Bacterial Amyloids In Vitro
Monitoring Molecular Assembly of Biofilms Using Quartz Crystal Microbalance with Dissipation (QCM-D)
Measuring the Size and Spontaneous Fluctuations of Amyloid Aggregates with Fluorescence Correlation Spectroscopy
Determining the Stoichiometry of Amyloid Oligomers by Single Molecule Photobleaching
Structural Information on Bacterial Amyloid and Amyloid-DNA Complex Obtained by Small-Angle Neutron/X-Ray Scattering
Fiber Diffraction and Small-Angle Scattering for Structural Investigation of Bacterial Amyloids
Supported Lipid Bilayers (SLBs) to Study Amyloid-Lipid Membrane Interactions with Atomic Force Microscopy
Characterization of Bacterial Amyloids by Nano-Infrared Spectroscopy
High Throughput Screening of Heterologous Functional Amyloids Using Escherichia coli
Evaluation of Amyloid Inhibitor Efficiency to Block Bacterial Survival
Anti-Amyloid Drug Screening Methods Using Bacterial Inclusion Bodies
Determination of Effects and Mechanisms of Action of Bacterial Amyloids on Antibiotic Resistance
Design and Testing of Synthetic Catalytic Amyloids Based on the Active Site of Enzymes
Analysis of Bacterial Amyloid Interaction with Lipidic Membrane by Orientated Circular Dichroism and Infrared Spectroscopies
Direct, Rapid, and Simple Evaluation of the Expression and Conformation of Beta-Amyloid in Bacterial Cells by FTIR Spectroscopy
Assessment of Intracellular Amyloid Formation in Fixed and Live Bacteria Using Fluorescence Microscopy
Identification of Aggregation-Prone and Gatekeeper Residues in Bacterial Amyloids Using Site-Directed Mutagenesis and Flow Cytometry
Probing DNA-Amyloid Interaction and Gel Formation by Active Magnetic Wire Microrheology
Probing Amyloids-DNA Interaction with Nanofluidics
Evaluation of the Role of Bacterial Amyloid on Nucleoid Structure Using Cryo Soft X-Ray Tomography.
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Bacterial amyloids : methods and protocols / edited by Véronique Arluison, Frank Wien, Andrés Marcoleta.