Details

Subject(s)

• Chromaffin cells [Browse]

Editor

• Borges, Ricardo [Browse]

Series

• Methods in molecular biology (Clifton, N.J.) ; v. 2565. [More in this series]
• Springer protocols (Series) [More in this series]
• Methods in molecular biology, 1064-3745 ; 2565
• Springer protocols

Summary note

This volume covers the most up-to-date methods and techniques used to further the understanding of chromaffin cell biology and pharmacology. Chapters guide readers through the basic mechanisms that regulate the stimulus-secretion coupling, chromaffin, tumor-derived cell PC-12 , morphology, biochemistry, pharmacology, electrophysiology, and electrochemistry. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Chromaffin Cells: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field. .

Bibliographic references

Includes bibliographical references and index.

Rights and reproductions note

Current copyright fee: GBP19.00 42\0.

Contents

• Rat pheochromocytoma PC12 cells in culture
• Adrenal grafts in the central nervous system: Chromaffin and chromaffin progenitor cell transplantation
• Immunocytochemistry of acutely isolated adrenal medullary chromaffin cells
• Transmission electron microscopy: a method for studying the adrenochromaffin cells
• Immunogold for protein location in chromaffin cells
• Visualization of exo- and endocytosis membrane dynamics with super-resolution STED microscopy
• Online detection of catecholamine release from the perfused rat adrenal gland
• On-line recording of perifused chromaffin cells
• Recording of chromaffin cell electrical activity in situ in acute adrenal slices
• Calcium imaging and amperometric recording in cultured chromaffin cells and adrenal slices from normotensive, Wistar Kyoto rats, and Spontaneously Hypertensive Rats
• Measurements of calcium in chromaffin cell organelles using targeted aequorins
• Quantification of secretory granule exocytosis by TIRF imaging and capacitance measurements
• Membrane capacitance measurements of stimulus-evoked exocytosis in adrenal chromaffin cell
• Quantal release analysis of electrochemically active molecules using single-cell amperometry
• Methodologies for detecting quantal exocytosis in adrenal chromaffin cells through diamond-based MEAs
• Vesicle collision protocols for the study of quantum size and exocytotic fraction released
• Patch Amperometry and Intracellular Patch Electrochemistry
• Artificial cells for dissecting exocytosis
• Isolation and purification of chromaffin granules from adrenal glands and cultured neuroendocrine cells
• Confocal microscopy studies of F-actin cytoskeleton distribution and dynamics using fluorescent LifeAct constructs in bovine adrenal chromaffin cells
• Unveiling the nanoscale dynamics of the exocytic machinery in chromaffin cells with single-molecule imaging
• Determination of catecholamines in a small volume (25[mu]l) of plasma from conscious mouse tail vein
• Quantification of chromogranin A and its fragments in biological fluids
• Cytotoxicity models in chromaffin cells to evaluate neuroprotective compounds.

Show 21 more Contents items

ISBN

• 9781071626702 ((hardback))
• 1071626701 ((hardback))

OCLC

1338673293

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