Chromaffin cells : methods and protocols / edited by Ricardo Borges.

New York, NY : Humana Press, [2023]
xiv, 373 pages : illustrations (some color) ; 26 cm.


Summary note
This volume covers the most up-to-date methods and techniques used to further the understanding of chromaffin cell biology and pharmacology. Chapters guide readers through the basic mechanisms that regulate the stimulus-secretion coupling, chromaffin, tumor-derived cell PC-12 , morphology, biochemistry, pharmacology, electrophysiology, and electrochemistry. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Chromaffin Cells: Methods and Protocols aims to be a useful practical guide to researches to help further their study in this field. .
Bibliographic references
Includes bibliographical references and index.
Rights and reproductions note
Current copyright fee: GBP19.00 42\0.
  • Rat pheochromocytoma PC12 cells in culture
  • Adrenal grafts in the central nervous system: Chromaffin and chromaffin progenitor cell transplantation
  • Immunocytochemistry of acutely isolated adrenal medullary chromaffin cells
  • Transmission electron microscopy: a method for studying the adrenochromaffin cells
  • Immunogold for protein location in chromaffin cells
  • Visualization of exo- and endocytosis membrane dynamics with super-resolution STED microscopy
  • Online detection of catecholamine release from the perfused rat adrenal gland
  • On-line recording of perifused chromaffin cells
  • Recording of chromaffin cell electrical activity in situ in acute adrenal slices
  • Calcium imaging and amperometric recording in cultured chromaffin cells and adrenal slices from normotensive, Wistar Kyoto rats, and Spontaneously Hypertensive Rats
  • Measurements of calcium in chromaffin cell organelles using targeted aequorins
  • Quantification of secretory granule exocytosis by TIRF imaging and capacitance measurements
  • Membrane capacitance measurements of stimulus-evoked exocytosis in adrenal chromaffin cell
  • Quantal release analysis of electrochemically active molecules using single-cell amperometry
  • Methodologies for detecting quantal exocytosis in adrenal chromaffin cells through diamond-based MEAs
  • Vesicle collision protocols for the study of quantum size and exocytotic fraction released
  • Patch Amperometry and Intracellular Patch Electrochemistry
  • Artificial cells for dissecting exocytosis
  • Isolation and purification of chromaffin granules from adrenal glands and cultured neuroendocrine cells
  • Confocal microscopy studies of F-actin cytoskeleton distribution and dynamics using fluorescent LifeAct constructs in bovine adrenal chromaffin cells
  • Unveiling the nanoscale dynamics of the exocytic machinery in chromaffin cells with single-molecule imaging
  • Determination of catecholamines in a small volume (25[mu]l) of plasma from conscious mouse tail vein
  • Quantification of chromogranin A and its fragments in biological fluids
  • Cytotoxicity models in chromaffin cells to evaluate neuroprotective compounds.
  • 9781071626702 ((hardback))
  • 1071626701 ((hardback))
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