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Princeton University Library Catalog
Alternative splicing : methods and protocols / edited by Peter Scheiffele and Oriane Mauger.
New York, NY : Humana Press, 
xi, 354 pages : illustrations (some color) ; 26 cm.
Methods in molecular biology (Clifton, N.J.) ; v. 2537.
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Springer protocols (Series)
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Methods in molecular biology, 1064-3745 ; 2537
This detailed volume collects commonly used and cutting-edge methods to analyze alternative splicing, a key step in gene regulation. After an introduction of the alternative splicing mechanism and its targeting for therapeutic strategies, the book continues with techniques for analyzing alternative splicing profiles in complex biological systems, visualizing and localizing alternative spliced transcripts with cellular and sub-cellular resolution, probing regulators of alternative splicing, as well as assessing the functional consequences of alternative splicing. Written for the highly successful Methods in Molecular Biology series, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Alternative Splicing: Methods and Protocols serves as an ideal guide for both RNA aficionados that want to implement novel approaches in their labs and novices undertaking alternative splicing projects.
Includes bibliographical references and index.
Alternative Splicing in Human Biology and Disease
Targeting Alternative Splicing for Therapeutic Interventions
An Optimized Protocol for the Mapping of Cell Type-Specific Ribosome-Associated Transcript Isoforms from Small Mouse Brain Regions
FACS-Based Neuronal Cell Type-Specific RNA Isolation and Alternative Splicing Analysis
Quantitative Measurement of Alternatively Spliced RNA Isoform Levels
Analysis of Splicing Regulation by Third-Generation Sequencing
Computational Analysis of Alternative Splicing Using VAST-TOOLS and the VastDB Framework
Identification and Quantification of Microexons Using Bulk and Single-Cell RNA-Seq Data
Functional Annotation of Custom Transcriptomes
Design, Labeling, and Application of Probes for RNA smFISH
BaseScope"!Approach to Visualize Alternative Splice Variants in Tissue
In Situ Imaging of mRNA Splicing Variants by SpliceRCA
Two-Color Fluorescent Reporters for Analysis of Alternative Splicing
Quantitative Detection of Protein Splice Variants by Selected Reaction Monitoring (SRM) Mass Spectrometry
Probing the Interactions of Splicing Regulatory Small Molecules and Proteins with U1 snRNP Using NMR Spectroscopy
Genome-Wide CRISPR Screening to Identify Mammalian Factors that Regulate Intron Retention
Tethered Function Assays to Elucidate the Role of RNA-Binding Proteins
Probing Liquid-Liquid Phase Separation of RNA-Binding Proteins In Vitro and In Vivo
Analysis of Oligonucleotide Biodistribution and Metabolization in Experimental Animals.
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Alternative Splicing [electronic resource] : Methods and Protocols / edited by Peter Scheiffele, Oriane Mauger.