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DNA Electrophoresis [electronic resource] : Methods and Protocols / edited by Svetlana Makovets.
Format
Book
Language
English
Εdition
1st ed. 2013.
Published/Created
Totowa, NJ : Humana Press : Imprint: Humana, 2013.
Description
1 online resource (X, 299 p. 56 illus., 20 illus. in color.)
Availability
Available Online
Springer Nature - Complete Protocols
Details
Subject(s)
Human genetics
[Browse]
Electrophoresis
[Browse]
Editor
Makovets, Svetlana
[Browse]
Makovets, Svetlana
[Browse]
Makovets, Svetlana
[Browse]
Series
Methods in Molecular Biology, 1054
[More in this series]
Methods in Molecular Biology, 1064-3745 ; 1054
[More in this series]
Summary note
In DNA Electrophoresis: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study DNA using electrophoresis as the major approach. A powerful tool that allows separating DNA molecules according to their size and shape, this volume includes methods and techniques such as 2-dimentional gel electrophoresis as the major approach. These include methods and techniques such as 2-dimentional gel electrophoresis, DNA electrophoresis under conditions in which DNA molecules are completely or partially denatured during the runs, Pulse Field Gel Electrophoresis, electrophoresis coupled to fluorescence in situ hybridization, as well as protein-DNA interactions studied using electrophoreses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, DNA Electrophoresis: Methods and Protocols aids scientists in continuing to study DNA dynamics both in live cells and in test tubes.
Notes
Bibliographic Level Mode of Issuance: Monograph
Language note
English
Contents
DNA Electrophoresis: Historical and Theoretical Perspectives
Basic DNA Electrophoresis in Molecular Cloning: a Comprehensive Guide for Beginners
Analysis of Branched DNA Replication and Recombination Intermediates from Prokaryotic Cells by Two-dimensional (2D) Native-native Agarose Gel Electrophoresis
Analysis of DNA Structures from Eukaryotic cells by Two-dimensional Native-native DNA Agarose Gel Electrophoresis
Directionality of Replication Fork Movement Determined by Two-dimensional Native-native DNA Agarose Gel Electrophoresis
Native/Denaturing Two-Dimensional DNA Electrophoresis And Its Application To The Analysis Of Recombination Intermediates
Plasmid DNA Topology Assayed by Two-dimensional Agarose Gel Electrophoresis
A Neutral Glyoxal Gel Electrophoresis Method for the Detection and Semi-Quantitation of DNA Single-Strand Breaks
Denaturing Gradient Gel Electrophoresis (DGGE)
Polyacrylamide Temperature Gradient Gel Electrophoresis
Separation of DNA Oligonucleotides using Denaturing Urea-PAGE
Pulsed-field Gel Electrophoresis of Bacterial Chromosomes
Resolution of Budding Yeast Chromosomes using Pulse Field Gel Electrophoresis
Analysis of DNA Damage Via Single Cell Electrophoresis
Fluorescence in situ Hybridisation on Electrophoresed Cells to Detect Sequence Specific DNA Damage
Analysis of Protein-DNA Interactions using PAGE: Band-shift Assays
Assaying Cooperativity of Protein-DNA Interactions using Agarose Gel Electrophoresis
DNA Bending by Proteins: Utilizing Plasmid pBendAT as a Tool
Using PCR coupled to PAGE for Detection and Semi-quantitative Evaluation of Telomerase Activity.
Show 16 more Contents items
ISBN
1-62703-565-6
Doi
10.1007/978-1-62703-565-1
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Princeton University Library aims to describe library materials in a manner that is respectful to the individuals and communities who create, use, and are represented in the collections we manage.
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DNA electrophoresis : methods and protocols / edited by Svetlana Makovets.
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