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Kidney Organogenesis [electronic resource] : Methods and Protocols / edited by Seppo Vainio.
1st ed. 2019.
New York, NY : Springer New York : Imprint: Humana, 2019.
1 online resource (xi, 248 pages) : illustrations (some color)
Methods in Molecular Biology, 1926
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Methods in Molecular Biology, 1064-3745 ; 1926
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This book details protocols on classical methods and very modern ex vivo mammalian developmental kidney model systems. Chapters focus on generation of mosaic embryonic kidneys, culture of the kidney tissues ex vivo, embryonic kidney progenitor cell, 3Dkidney model cell line based cultures, programming the kidney cell fates in human pluripotent cells, protocols to target the functional players in kidney ontogenesis, 4D and 3D ex vivo imaging, drosphila, and computational approaches to kidney ontogenesis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Kidney Organogenesis: Methods and Protocols aims to ensure successful results in the further study of this vital field.
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Generating Genetic Mosaic Mouse Embryos or Organoids for Studies of Kidney Development
Mouse Ex Vivo Kidney Culture Methods
Serum-Free Organ Culture of the Embryonic Mouse Ureter
Experimental Tubulogenesis Induction Model in the Mouse
Dissociation of Tissues for Single Cell Analysis
Long Term Culture of Nephron Progenitor Cells Ex Vivo
3D Cell Culture Models of Epithelial Tissues
Generation of Three-Dimensional Nephrons from Mouse and Human Pluripotent Stem Cells
Generating Multiple Kidney Progenitors and Cell Types from Human Pluripotent Stem Cells
Protocol to Generate Ureteric Bud Structures from Human iPS Cells
Investigating Aspects of Renal Physiology and Pharmacology in Organ and Organoid Culture
Virus as Renal Functional Genetic Tools
Gene-Editing in 3D Cultured Nephron Progenitor Cell Lines
Time-Lapse Technologies and 4D Imaging of Kidney Development
Decellularizing and Recellularizing Adult Mouse Kidneys
Optical Projection Tomography Imaging to Study Kidney Organogenesis
Epithelial Function in the Drosophila Malpighian Tubule: An In Vivo Renal Model
Analysis of the Teashirt Target Genes in Ureteric Bud Development
Methods of Computational Analysis in Kidney Development.
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Kidney organogenesis : methods and protocols / edited by Seppo Vainio.