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MicroRNAs in Development [electronic resource] : Methods and Protocols / edited by Tamas Dalmay.
Format
Book
Language
English
Εdition
1st ed. 2011.
Published/Created
Totowa, NJ : Humana Press : Imprint: Humana, 2011.
Description
1 online resource (X, 200 p. 36 illus., 2 illus. in color.)
Availability
Available Online
SpringerNature Complete Protocols
Online Content
Online Content
Details
Subject(s)
Cytology
[Browse]
Gene expression
[Browse]
Editor
Dalmay, Tamas
[Browse]
Dalmay, Tamas
[Browse]
Dalmay, Tamas
[Browse]
Series
Methods in Molecular Biology, 732
[More in this series]
Methods in Molecular Biology, 1064-3745 ; 732
[More in this series]
Subseries of
Methods in molecular biology
Summary note
Since the discovery of microRNAs, developmental biologists have striven to understand the role of miRNAs in development and disease. MicroRNAs in Development: Methods and Protocols collects contributions from expert researchers in order to provide practical guidelines to this complex study. Divided into three convenient sections, this detailed volume covers various techniques to detect and profile miRNA expression, followed by protocols to manipulate the activity of miRNAs in various organisms, and it concludes with a section that outlines different methods to identify and validate miRNA targets in animals and plants. Written in the highly successful Methods in Molecular Biology™ series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, MicroRNAs in Development: Methods and Protocols serves as a practical guide for scientists of all backgrounds and conveys the appropriate sense of fascination associated with this vital field of research.
Notes
Bibliographic Level Mode of Issuance: Monograph
Language note
English
Contents
In situ Detection of microRNAs in Animals
Detection of microRNAs in Plants by In situ Hybridization
Detecting Animal and Plant microRNAs by Northern Blotting
Profiling microRNAs by Real-time PCR
Detection of Small RNAs and microRNAs using Deep Sequencing Technology
Detection of microRNAs in Prostate Cancer Cells by microRNA Array
microRNA Knock Down by Cholesterol Conjugated Antisense Oligos in Mouse Organ Culture
Protocols for Use of Homologous Recombination Gene-targeting to Produce microRNA Mutants in Drosophila
Engineering Elements for Gene Silencing: The Artificial microRNAs Technology
Mimicry Technology: Suppressing Small RNA Activity in Plants
Experimental Validation of MicroRNA Targets using a Luciferase Reporter System
Experimental Identification of microRNA Targets by Immunoprecipitation of Argonaute Protein Complexes
Comprehensive Identification of miRNA Target Sites in Live Animals
Target Validation of Plant microRNAs.- A High-throughput Sequencing-based Methodology to Identify All Uncapped and Cleaved RNA Molecules in Eukaryotic Genomes.
Show 11 more Contents items
ISBN
1-61779-083-4
Doi
10.1007/978-1-61779-083-6
Statement on language in description
Princeton University Library aims to describe library materials in a manner that is respectful to the individuals and communities who create, use, and are represented in the collections we manage.
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MicroRNAs in development [electronic resource] : methods and protocols / edited by Tamas Dalmay.
id
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MicroRNAs in development : methods and protocols / edited by Tamas Dalmay.
id
9965502493506421