In vitro bioassay techniques for anticancer drug discovery and development / Dhanya Sunil, Pooja R. Kamath and H. Raghu Chandrashekhar.

Author
Sunil, Dhanya [Browse]
Format
Book
Language
English
Published/​Created
Boca Raton : CRC Press, [2017]
Description
1 online resource (x, 105 pages) : illustrations

Availability

Details

Subject(s)
Author
Library of Congress genre(s)
Series
Online access with DDA: Askews (Medicine) [More in this series]
Summary note
This comprehensive and useful handbook represents a definitive up-to-date compendium of key in vitro bioassay methods that are employed to quantify and validate the anticancer activity of a drug candidate before it makes its way in to animal or clinical trials. In Vitro Bioassay Techniques for Anticancer Drug Discovery and Development covers the screening and evaluation of potential drug candidates in a wide category of anticancer assays demonstrating the specific ways in which various pharmaceutical bioassays interpret the activity of drug molecules. The major emphasis of the book is to present those bioassays which can be readily set up and practiced in any laboratory with limited funds, facilities or technical know-how.
Bibliographic references
Includes bibliographical references and index.
Source of description
Print version record.
Contents
  • Cover ; Half Title ; Title Page ; Copyright Page ; Contents ; Preface ; Chapter 1: Introduction to Anticancer Drug Development ; 1.1 Cancer ; 1.2 Chemotherapy ; 1.3 Need for Effective Anticancer DrugS ; 1.4 Stages of Anticancer Drug Development ; References.
  • Chapter 2: In Vitro Biological Assays for Anticancer Chemotherapeutics 2.1 Introduction ; 2.2 Advantages of in Vitro Assays ; 2.3 Disadvantages of in Vitro Assays ; 2.4 Classification of in Vitro Assays ; 2.5 Essential Techniques of Cell Cultures ; 2.5.1 Primary Cell Cultures.
  • 2.5.2 Maintenance of Cell Lines 2.5.3 Subculture of Cells ; References ; Chapter 3: Cell Viability and Cytotoxicity Assays ; 3.1 Introduction ; 3.2 Cell Membrane Integrity Assays ; 3.2.1 Dye Exclusion Assays ; 3.2.1.1 Trypan Blue Assay.
  • 3.2.1.2 Calcein Acetoxymethyl (Calcein-AM) Assay 3.2.1.3 Propidium Iodide (PI)/7-Aminoactinomycin D (7-AAD) Staining Assay ; 3.2.2 LDH Release Assays ; 3.3 Functional or Metabolic Assays ; 3.3.1 Tetrazolium Reduction Assays ; 3.3.1.1 MTT Assay ; 3.3.1.2 MTS Assay ; 3.3.1.3 XTT Assay.
  • 3.3.1.4 WST Assay 3.3.2 Sulforhodamine B Assay ; 3.3.3 Acid Phosphatase Assay ; 3.3.4 Alamar Blue Oxidation-Reduction Assay ; 3.3.5 Neutral Red Assay ; 3.3.6 Protease Viability Marker Assay ; 3.4 Cell Adhesion Assay ; References ; Chapter 4: Cell Proliferation Assays; 4.1 Introduction.
ISBN
  • 9781351751537 ((electronic bk.))
  • 1351751530 ((electronic bk.))
  • 9781351751520 ((electronic bk.))
  • 1351751522 ((electronic bk.))
  • 9781315191256 ((electronic bk.))
  • 1315191253 ((electronic bk.))
OCLC
988175658
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