Details

Subject(s)

• CRISPR (Genetics) [Browse]
• Genetic engineering [Browse]
• Genomics [Browse]
• Endonucleases [Browse]
• Restriction enzymes, DNA [Browse]

Editor

• Bailey, Scott [Browse]

Series

Methods in enzymology ; vol. 616. [More in this series]

Summary note

CRISPR-Cas Enzymes, Volume 616, the latest release in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Topics covered in this release include CRISPR bioinformatics, A method for one-step assembly of Class 2 CRISPR arrays, Biochemical reconstitution and structural analysis of ribonucleoprotein complexes in Type I-E CRISPR-Cas systems, Mechanistic dissection of the CRISPR interference pathway in Type I-E CRISPR-Cas system, Site-specific fluorescent labeling of individual proteins within CRISPR complexes, Fluorescence-based methods for measuring target interference by CRISPR-Cas systems, Native State Structural Characterization of CRISRP Associated Complexes using Mass Spectrometry, and more.

Bibliographic references

Includes bibliographical references.

Contents

• Predicting and visualizing features of CRISPR-Cas systems / Matthew A. Nethery and Rodolphe Barrangou
• Reconstitution and biochemical characterization of ribonucleoprotein complexes in Type I-E CRISPR-Cas systems / Yibei Xiao and Ailong Ke
• Sortase-mediated fluorescent labeling of CRISPR complexes / Kaylee E. Dillard, Jeffrey M. Schaub, Maxwell W. Brown, Fatema A. Saifuddin, Yibei Xiao, Erik Hernandez, Samuel D. Dahlhauser, Eric V. Anslyn, Ailong Ke and Ilya J. Finkelstein
• Fluorescence-based methods for measuring target interference by CRISPR-Cas systems / Phong T. Phan, Michael Schelling, Chaoyou Xue and Dipali G. Sashital
• Probing Cascade complex composition and stability using native mass spectrometry techniques / Angela Patterson, Monika Tokmina-Lukaszewska and Brian Bothner
• High-throughput determination of in vivo DNA sequence preferences for Cas protein binding using Library-ChIP / Joseph T. Wade
• Live-cell single-particle tracking photoactivated localization microscopy of Cascade-mediated DNA surveillance / Bartosz Turkowyd, Hanna Müller-Esparza, Vanessa Climenti, Niklas Steube, Ulrike Endesfelder and Lennart Randau
• In vitro assembly of thermostable Csm complex in CRISPR-Cas type III/A system / Kwang-Hyun Park, Yan An and Eui-Jeon Woo
• Investigation of the cyclic oligoadenylate signaling pathway of type III CRISPR systems / Christophe Rouillon, Januka S. Athukoralage, Shirley Graham, Sabine Grüschow and Malcolm F. White
• A pipeline for characterization of novel Cas9 orthologs / Tautvydas Karvelis, Joshua K. Young and Virginijus Siksnys
• Preparation and electroporation of Cas12a/Cpf1-guide RNA complexes for introducing large gene deletions in mouse embryonic stem cells / Lucas Kissling, Asun Monfort, Daan C. Swarts, Anton Wutz and Martin Jinek
• Directed evolution studies of a thermophilic Type II-C Cas9 / Travis H. Hand, Anuska Das and Hong Li
• Kinetic characterization of Cas9 enzymes / Mu-Sen Liu, Shanzhong Gong, Helen-Hong Yu, David W. Taylor and Kenneth A. Johnson
• Single-molecule FRET studies of Cas9 endonuclease / Viktorija Globyte and Chirlmin Joo
• CRISPR-Cas molecular beacons as tool for studies of assembly of CRISPR-Cas effector complexes and their interactions with DNA / Vladimir Mekler, Konstantin Kuznedelov, Leonid Minakhin, Karthik Murugan, Dipali G. Sashital and Konstantin Severinov
• Adapting dCas9-APEX2 for subnuclear proteomic profiling / Xin D. Gao, Tomás C. Rodríguez and Erik J. Sontheimer
• CRISPR-Cas10 assisted editing of virulent staphylococcal phages / S.M. Nayeemul Bari and Asma Hatoum-Aslan
• Reconstitution of CRISPR adaptation in vitro and its detection by PCR / Robert D. Fagerlund, Timothy J. Ferguson, Howard W.R. Maxwell, Helen K. Opel-Reading, Kurt L. Krause and Peter C. Fineran.

Show 15 more Contents items

ISBN

• 0128167602 (hardcover)
• 9780128167601 (hardcover)

OCLC

1097075809

Statement on language in description

Princeton University Library aims to describe library materials in a manner that is respectful to the individuals and communities who create, use, and are represented in the collections we manage. Read more...