Plant transcription factors : methods and protocols / edited by Ling Yuan, Sharyn E. Perry.

Format
Book
Language
English
Published/​Created
New York : Humana, ©2011.
Description
xi, 347 pages : illustrations (some color) ; 27 cm

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Available Online

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Lewis Library - Lewis Library - Serials (Off-Site) QH506 .M45 1984 vol.754 Browse related items Request

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    Summary note
    [Publisher-supplied data] Recent years have seen significant advancements in the development of enabling technologies that facilitate the study of Transcription Factors (TFs). TFs are pivotal in the regulation of plant development, reproduction, intercellular signaling, response to environment, cell cycle, and metabolism. Plant Transcription Factors: Methods and Protocols offers a comprehensive approach by covering the basic concepts as well as the detailed protocols of a series of commonly used tools for investigating plant TFs. From discussing select TF families in plants to presenting approaches for identifying them, methods are covered to verify the function, to identify protein interactions in which TFs are involved, and how the interactions are mediated. Increasing examples of TFs that function non-cell-autonomously are being discovered and methods to assess intercellular trafficking are also addressed. A section is devoted to examining interaction with DNA, and the volume concludes with a discussion of directed evolution to generate transcription factors that can more efficiently control desired processes. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Plant Transcription Factors: Methods and Protocols serves as an ideal guide to seasoned plant molecular biologists as well as scientists new to the field of TFs and provides many necessary methods to all scientists who are interested in exploring the functions of transcription factors.
    Bibliographic references
    Includes bibliographical references and index.
    Contents
    • 1. MADS and more: transcription factors that shape the plant / Rainer Melzer and Günter Theißen
    • 2. In silico mining and PCR-based approaches to transcription factor discovery in non-model plants: gene diversity of the WRKY transcription factors in conifers / Jun-Jun Liu and Yu Xiang
    • 3. Isolation of plant transcription factors using a yeast one-hybrid system / Tatiana Pyvovarenko and Sergiy Lopato
    • 4. A transposon-based activation tagging system for gene function discovery in arabidopsis / Nayelli Marsch-Martinez
    • 5. CRWA-T, an effective gene silencing system utilizing chimeric repressors / Nobutaka Mitsuda, Kyoko Matsui, Miho Ikeda, Masaru Nakata, Yoshimi Oshima, Yukari Nagatoshi, and Masaru Ohme-Takagi
    • 6. Analysis of a transcription factor using transient assay in arabidopsis protoplasts / Yuji Iwata, Mi-Hyun Lee, and Nozomu Koizumi
    • 7. Microarray-based identification of transcription factor target genes / Maartje Gorte, Anneke Horstman, Robert B. Page, Renze Heidstra, Arnold Stromberg and Kim Boutilier
    • 8. Yeast protein-protein interaction assays and screens / Stefan de Folter and Richard G.H. Immink
    • 9. Mapping functional domains of transcription factors / Ling Zhu and Enamul Huq
    • 10. Biomolecular fluorescence complementation as a tool to study interactions of regulatory proteins in plant protoplasts / Sitakanta Pattanaik, Joshua R. Werkman, and Ling Yuan
    • 11. Isolation of transcription factor complexes from arabidopsis cell suspension cultures by tandem affinity purification / Jelle Van Leene, Dominique Eeckhout, Geert Persiau, Eveline Van De Slijke, Jan Geerinck, Gert Van Isterdael, Erwin Witters, and Geert De Jaeger
    • 12. Assaying transcription factor stability / Jasmina Kurepa and Jan A. Smalle
    • 13. How to access the intercellular trafficking of transcription factors / Munawar Ahmad, Won Kyong Cho, Yeonggil Rim, Lijun Huang, and Jae-Yean Kim
    • 14. SELEX (systematic evolution of ligands by exponential enrichment), as a powerful tool for deciphering the protein-DNA interaction space / Chenglin Chai, Zidian Xie, and Erich Grotewold
    • 15. Footprinting and missing nucleoside analysis of transcription factor-DNA complexes / Ivana L. Viola and Daniel H. Gonzalez
    • 16. Chromatin immunoprecipitation to verify or to identify in vivo protein-DNA interactions / Yumei Zheng and Sharyn E. Perry
    • 17. Visualizing and characterizing invivo DNA-binding events and direct target genes of plant transcription factors / Jose M. Muiño, Gerco C. Angenent, and Kerstin Kaufmann
    • 18. Mapping in vivo protein-DNA interactions in plants by DamID, a DNA adenine methylation-based method / Sophie Germann and Valérie Gaudin
    • 19. Directed evolution through DNA shuffling for the improvement and understanding of genes and promoters / Joshua R. Werkman, Sitakanta Pattanaik, and Ling Yuan.
    Other format(s)
    Also available in an electronic version.
    ISBN
    • 1617791539 ((alk. paper))
    • 9781617791536 ((alk. paper))
    • 9781617791543 ((e-ISBN))
    • 1617791547 ((e-ISBN))
    LCCN
    2011931514
    OCLC
    723107867
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