Plant transcription factors [electronic resource] : methods and protocols / edited by Ling Yuan, Sharyn E. Perry.

Format
Book
Language
English
Published/​Created
New York : Humana Press, c2011.
Description
1 online resource (xi, 347 p.) : ill.

Availability

Available Online

Details

Subject(s)
Series
  • Methods in molecular biology (Clifton, N.J.) ; v. 754. [More in this series]
  • Methods in molecular biology, 1940-6029 ; 754
Bibliographic references
Includes bibliographical references and index.
Source of description
Description based on print version record.
Contents
  • MADS and more: Transcription factors that shape the plant
  • In silico mining and PCR-based approaches to transcription factor discovery in non-model plants: Gene discovery of the WRKY transcription factors in conifers
  • Isolation of plant transcription factors using a yeast one-hybrid system
  • A transposon-based activation tagging system for gene function discovery in arabidopsis
  • CRES-T, an effective gene silencing system utilizing chimeric repressors
  • Analysis of a transcription factor using transient assay in arabidopsis protoplasts
  • Microarray-based identification of transcription factor target genes
  • Yeast protein-protein interaction assays and screens
  • Mapping functional domains of transcription factors
  • Bimolecular fluorescence complementation as a tool to study interactions of regulatory proteins in plant protoplasts
  • The hepatitis nucleocapsid as a vaccine carrier moiety
  • Isolation of transcription factor complexes from arabidopsis cell suspension cultures by tandem affinity purification
  • Assaying transcription factor stability
  • How to assess the intercellular trafficking of transcription factors
  • SELEX (systematic evolution of ligands by exponential enrichment), as a powerful tool for deciphering the protein-DNA interaction space
  • Footprinting and missing nucleoside analysis of transcription factor-DNA complexes
  • Chromatin immunoprecipitation to verify or to identify in vivo protein-DNA interactions
  • Visualizing and characterizing in vivo DNA-binding events and direct target genes of plant transcription factors
  • Mapping in vivo protein-DNA interactions in plants by damid, a DNA adenine methylation-based method
  • Directed evolution through DNA shuffling for the improvement and understanding of genes and promoters
  • Thiophilic interaction chromatography of prostate-specific antigen.
ISBN
  • 9781617791543 (e-ISBN)
  • 1617791547 (e-ISBN)
OCLC
740821635
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