RNA detection and visualization : methods and protocols / edited by Jeffrey E. Gerst.

Format
Book
Language
English
Published/​Created
New York : Humana Press, ©2011.
Description
xiv, 497 pages : illustrations (some color) ; 27 cm

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Lewis Library - Lewis Library - Serials (Off-Site) QH506.M45 1984 vol.714 Browse related items Request

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    Summary note
    With its complex and extensively regulated metabolism, the study of the RNA lifecycle demands tools that allow for the localization of RNAs to be observed either in an in situ setting or, preferably, under in vivo conditions. In RNA Detection and Visualization: Methods and Protocols, the best and brightest investigators provide an up-to-date and in-depth description of basic methods and protocols used for detecting and visualizing mRNAs in both fixed and live cells, from bacteria to mammals. For novices and experts alike, this mix of classic in situ hybridization and advanced live imaging techniques, cell fractionation and affinity purification procedures, and bioinformatics tools gives researchers the most complete and extensive array of research aids possible. As a volume written in the highly successful Methods in Molecular Biology"!series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, RNA Detection and Visualization: Methods and Protocols offers well-honed techniques in order to inspire researchers around the world to further our knowledge of the vital biological significance of RNA
    Bibliographic references
    Includes bibliographical references and index.
    Contents
    • Single molecule imaging of RNA in situ / Mona Batish, Arjun Raj, and Sanjay Tyagi
    • FISH and immunofluorescence staining in Chlamydomonas / James Uniacke, Daniel Colón-Ramos, and William Zerges
    • High resolution fluorescent in situ hybridization in Drosophila / Eric Lécuyer
    • Localization and anchorage of maternal mRNAs to cortical structures of ascidian eggs and embryos using high resolution in situ hybridization / Alexandre Paix, Janet Chenevert, and Christian Sardet
    • Visualization of mRNA localization in Xenopus oocytes / James A. Gagnon and Kimberly L. Mowry
    • Visualization of mRNA expression in the zebrafish embryo / Yossy Machluf and Gil Levkowitz
    • High-resolution fluorescence in situ hybridization to detect mRNAs in neuronal compartments in vitro and in vivo / Sharon A. Swanger, Gary J. Bassell, and Christina Gross
    • Localization of mRNA in vertebrate axonal compartments by in situ hybridization / José Roberto Sotelo-Silveira [and others]
    • Tiny molecular beacons for in vivo mRNA detection / Diana P. Bratu, Irina E. Catrina, and Salvatore A.E. Marras
    • Delivery of molecular beacons for live-cell imaging and analysis of RNA / Antony K. Chen [and others]
    • Genetically-encoded fluorescent probes for imaging endogenous mRNA in living cells / Takeaki Ozawa and Yoshio Umezawa
    • Visualization of induced RNA in single bacterial cells / Azra Borogovac and Natalia E. Broude
    • Visualizing mRNAs in fixed and living yeast cells / Franck Gallardo and Pascal Chartrand
    • In vivo visualization of RNA using the U1A-based tagged RNA system / Sunglan Chung and Peter A. Takizawa
    • Visualizing endogenous mRNAs in living yeast using m-TAG, a PCR-based RNA aptamer integration method, and fluorescence microscopy / Liora Haim-Vilmovsky and Jeffrey E. Gerst
    • Imaging mRNAs in living mammalian cells / Sharon Yunger and Yaron Shav-Tal
    • Using the mRNA-MS2/MS2CP-FP system to study mRNA transport during Drosophila oogenesis / Katsiarina Belaya and Daniel St Johnston
    • Genome-wide analysis of RNA extracted from isolated mitochondria / Erez Eliyahu, Daniel Melamed and Yoav Arava
    • Analyzing mRNA localization to the endoplasmic reticulum via cell fractionation / Sujatha Jagannathan, Christine Nwosu and Christopher V. Nicchitta
    • Isolation of mRNAs encoding peroxisomal proteins from yeast using a combined cell fractionation and affinity purification procedure / Gadi Zipor, Cecile Brocard and Jeffrey E. Gerst
    • Profiling axonal mRNA transport / Dianna E. Willis and Jeffery L. Twiss
    • RNA purification from tumor cell protrusions using porous polycarbonate filters / Jay Shankar and Ivan R. Nabi
    • RNA-binding protein immunopurification-microarray (RIP-chip) analysis to profile localized RNAs / Alessia Galgano and André P. Gerber
    • RaPID : an aptamer-based mRNA affinity purification technique for the identification of RNA and protein factors present in ribonucleoprotein complexes / Boris Slobodin and Jeffrey E. Gerst
    • RIP : an mRNA localization technique / Sabarinath Jayaseelan [and others]
    • The dual use of RNA aptamer sequences for affinity purification and localization studies of RNAs and RNA-protein complexes / Scott C. Walker [and others]
    • Identifying and searching for conserved RNA localisation signals / Russell S. Hamilton and Ilan Davis
    • Computational prediction of RNA structural motifs involved in post-transcriptional regulatory processes / Michal Rabani, Michael Kertesz, and Eran Segal.
    Other format(s)
    Also available in an electronic version.
    ISBN
    • 9781617790041 ((alk. paper))
    • 1617790044 ((alk. paper))
    • 9781617790058 ((e-ISBN))
    • 1617790052 ((e-ISBN))
    LCCN
    2011924684
    OCLC
    690089446
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