Details

Subject(s)

• Cellular signal transduction [Browse]
• Immunohistochemistry [Browse]
• Immunocytochemistry [Browse]

Related name

• Kalyuzhny, Alexander E. [Browse]

Series

• Methods in molecular biology (Clifton, N.J.) ; v. 717. [More in this series]
• Springer protocols (Series) [More in this series]
• Methods in molecular biology, 1064-3745 ; 717
• Springer protocols

Summary note

[Publisher-supplied data] Unlike detecting constitutively expressed targets, immunohistochemical detection of labile, low abundance, and short-lived signal transduction molecules can be a very difficult task. In Signal Transduction Immunohistochemistry: Methods and Protocols, IHC experts contribute detailed protocols addressing the numerous challenges of signal-transduction immunohistochemistry (ST-IHC). Beginning with a set of introductory chapters, the volume moves on to cover techniques used for the preservation of antigens and their unmasking, protocols in digital imaging and image analysis of stained cells and tissues, high-throughput data collection and data analysis, and techniques used in neuroscience as well as cancer and stem cell research. Written in the highly successful Methods in Molecular Biology series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Signal Transduction Immunohistochemistry: Methods and Protocols serves as an ideal guide for novices and as a bastion of inspiring ideas to be exploited by experienced researchers on the lookout for new experimental tricks and hints.

Bibliographic references

Includes bibliographical references and index.

Contents

• Overview of the generation, validation, and application of phosphosite-specific antibodies / Kathy Brumbaugh ... [et al.]
• Selection and validation of antibodies for signal transduction immunohistochemistry / Juraj Bodo and Eric D. Hsi
• Overview of western blotting for determining antibody specificities for immunohistochemistry / Biji T. Kurien
• Optimized protocol to make phospho-specific antibodies that work / Amy J. Archuleta ... [et al.]
• Methodology and technology for stabilization of specific states of signal transduction proteins / Mats Boren
• Enhanced antigen-retrieval protocol for immunohistochemical staining of formalin-fixed, paraffin-embedded tissues / Sergei I. Syrbu and Michael B. Cohen
• Imaging techniques in signal transduction IHC / Jerry Sedgewick
• Practical considerations of image analysis and quantification of signal transduction IHC staining / Michael Grunkin, Jakob Raundahl, and Niels T. Foged
• Flow cytometric analysis of cell signaling proteins / Maria A. Suni and Vernon C. Maino
• CytoSys : a tool for extracting cell-cycle-related expression dynamics from static data / Jayant Avva ... [et al.]
• Signaling events initiated by kappa opioid receptor activation : quantification and immunocolocalization using phospho-selective KOR, p38 MAPK, and KIR 3.1 antibodies / Julia C. Lemos, Clarisse A. Roth, and Charles Chavkin
• Immunohistochemical assessment of signal transduction and cell-cycle networks in neural tumors / Daniel Ciznadija, Afsar Barlas, and Katia Manova
• Novel multicolor immunofluorescence technique using primary antibodies raised in the same host species / Jillian Frisch ... [et al.]
• Activation and differentiation of mesenchymal stem cells / Pravin J. Mishra and Debabrata Banerjee
• Double in situ detection of sonic hedgehog mRNA and pMAPK protein in examining the cell proliferation signaling pathway in mouse embryo / Sho Fujisawa ... [et al.]
• Identifying intracellular sites of eicosanoid lipid mediator synthesis with eicosacell assays / Christianne Bandeira-Melo, Peter F. Weller, and Patricia T. Bozza
• Absorption control in immunohistochemistry using phospho-peptides immobilized on magnetic beads / Jordan Schoephoerster ... [et al.].

Show 14 more Contents items

Other format(s)

Also available in an electronic version.

ISBN

• 9781617790232 ((alk. paper))
• 1617790230 ((alk. paper))
• 9781617790249 ((e-ISBN))
• 1617790249 ((e-ISBN))

LCCN

2011921260

OCLC

690089416

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