Live cell imaging : methods and protocols / edited by Dmitri B. Papkovsky.

Format
Book
Language
English
Published/​Created
New York, NY : Humana Press, ©2010.
Description
xi, 367 p. : ill.

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Lewis Library - Lewis Library - Serials (Off-Site) QH506 .M45 1984 vol.591 Browse related items Request

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    Summary note
    Now a routine tool in biomedical and life science research, live cell imaging has made major progress enabling this core biochemical, cell, and molecular biology technique to become even more powerful, versatile, and affordable. In Live Cell Imaging: Methods and Protocols, a panel of expert contributors provide a comprehensive compendium of experimental approaches to live cell imaging in the form of several overview chapters followed by representative examples and case studies covering different aspects of the most current methodology. By examining a range of state-of-the-art protocols extensively validated in complex biological studies, this volume highlights new experimental and instrumental opportunities and helps researchers to select appropriate imaging methods for their specific biological questions and measurement tasks.
    Bibliographic references
    Includes bibliographical references and index.
    Contents
    • Instrumentation for live-cell imaging and main formats / Martin Oheim
    • Labels and probes for live cell imaging : overview and selection guide / Scott A. Hilderbrand
    • Live cell imaging : an industrial perspective / Terry McCann
    • Design of fluorescent fusion protein probes / Elizabeth Pham and Kevin Truong
    • Synthetic fluorescent probes for imaging of peroxynitrite and hypochlorous acid in living cells / Dan Yang, Zhen-Ning Sun, Tao Peng, Hua-Li Wang, Jian-Gang Shen, Yan Chen, and Paul Kwong-Hang Tam
    • Photo-activatable probes for the analysis of receptor function in living cells / Wen-Hong Li
    • The application of fluorescent probes for the analysis of lipid dynamics during phagocytosis / Ronald S. Flannagan and Sergio Grinstein
    • Imaging of mitotic cell division and apoptotic intra-nuclear processes in multicolor / Kenji Sugimoto and Shigenobu Tone
    • Manipulation of neutrophil-like HL-60 cells for the study of directed cell migration / Arthur Millius and Orion D. Weiner
    • A method for analyzing protein-protein interactions in the plasma membrane of live B cells by fluorescence resonance energy transfer imaging as acquired by total internal reflection fluorescence microscopy / Hae Won Sohn, Pavel Tolar, Joseph Brzostowski, and Susan K. Pierce
    • Sample preparation for STED microscopy / Christian A. Wurm, Daniel Neumann, Roman Schmidt, Alexander Egner, and Stefan Jakobs
    • Two-photon permeabilization and calcium measurements in cellular organelles / Oleg Gerasimenko and Julia Gerasimenko
    • Imaging and analysis of three-dimensional cell culture models / Benedikt W. Graf and Stephen A. Boppart
    • Long-term imaging in microfluidic devices / Gilles Charvin, Catherine Oikonomou, and Frederick Cross
    • Monitoring of cellular responses to hypoxia / Christoph Wotzlaw and Joachim Fandrey
    • Imaging of cellular oxygen and analysis of metabolic responses of mammalian cells / Andreas Fercher, Tomas C. O'Riordan, Alexander V. Zhdanov, Ruslan I. Dmitriev, and Dmitri B. Papkovsky
    • Analysis of mitochondrial pH and ion concentrations / Martin vandeVen, Corina Balut, Szilvia Baron, Ilse Smets, Paul Steels, and Marcel Ameloot
    • Live cell imaging analysis of receptor function / Daniel C. Worth and Maddy Parsons
    • Subcellular dynamic imaging of protein-protein interactions in live cells by bioluminescence resonance energy transfer / Julie Perroy
    • Quantitative analysis of membrane potentials / Manus W. Ward
    • Image correlation spectroscopy to define membrane dynamics / Jeremy Bonor, Anja Nohe.
    Other format(s)
    Also available in an electronic version.
    ISBN
    • 9781607614036 ((alk. paper))
    • 1607614030 ((alk. paper))
    • 9781607614043 ((e-ISBN))
    • 1607614049 ((e-ISBN))
    LCCN
    2009939132
    OCLC
    428029086
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