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In vitro mutagenesis protocols [electronic resource] / edited by Jeff Braman.
Format
Book
Language
English
Εdition
3rd ed.
Published/Created
[Totowa, N.J.] : Humana Press, c2010.
Description
xiv, 442 p. : ill.
Availability
Available Online
Online Content
SpringerNature Complete Protocols
Online Content
Details
Subject(s)
Mutagenesis
—
Methodology
[Browse]
Related name
Braman, Jeff
[Browse]
SpringerLink (Online service)
[Browse]
Series
Methods in molecular biology (Clifton, N.J.) ; v. 634.
[More in this series]
Springer protocols.
[More in this series]
Methods in molecular biology, 1064-3745 ; 634
Bibliographic references
Includes bibliographical references and index.
Reproduction note
Electronic reproduction. New York : Springer, 2010. Mode of access: World Wide Web. System requirements: Web browser. Title from title screen (viewed on Sep. 9, 2010). Access may be restricted to users at subscribing institutions.
Contents
Mutagenesis protocols in Saccharomyces cerevisiae by in vivo overlap extension / Mibuel Alcalde
In vitro mutagenesis of Brucella species / Thomas A. Ficht, Jianwu Pei, and Melissa Kahl-McDonagh
Random mutagenesis strategies for Campylobacter and Helicobacter species / Duncan J.H. Gaskin and Arnoud H.M. van Vliet
Mutagenesis of the repeat regions of herpesviruses cloned as bacterial artificial chromosomes / Yuguang Zhao and Venugopal Nair
An efficient protocol for VZV BAC-based mutagenesis / Zhen Zhang, Ying Huang, and Hua Zhu
A method for rapid genetic integration into Plasmodium falciparum utilizing mycobacteriophage Bxb1 integrase / Sophie H. Adjalley, Marcus C.S. Lee, and David A. Fidock
Random mutagenesis by error-prone PCR / Elizabeth O. McCullum ... [et al.]
A rapid and versatile PCR-based site-directed mutagenesis protocol for generation of mutations along the entire length of a cloned cDNA / Vincent Dammai
Rapid sequence scanning mutagenesis using in silico oligo design and the megaprimer PCR of whole plasmid method (MegaWhop) / Urich Krauss, Karl-Erich Jaeger, and Thorsten Eggert
Insertion and deletion mutagenesis by overlap extension PCR / Jehan Lee ... [et al.]
Targeted amplification of mutant strands for efficient site-directed mutagenesis and mutant screening / Lei Young and Qihan Dong
A modified inverse PCR procedure for insertion, deletion, or replacement of a DNA fragment in a target sequence and its application in the ligand interaction scan method for generation of ligand-regulated proteins / Oran Erster and Moti Liscovitch
Amplification of orthologous genes using degererate primers / Samya Chakravorty and jim O. Vigoreaux
Computational evaluation of protein stability change upon mutations / Shuangye Yin, Feng Ding, and Nikolay V. Dokholyan
Approaches for using animal models to identify loci that genetically interact with human disease-causing point mutations / Josef D. Franke
Using peptide loop insertion mutagenesis for the evolution of proteins / Christian Heinis and Kai Johnsson
Massive mutagenesis : high-throughput combinatorial site-directed mutagenesis / Julien Sylvestre
Directed in vitro evolution of reporter genes based on semi-rational design and high-throughput screening / Ai-Sheng Xiong ... [et al.]
Ribosome display for rapid protein evolution by consecutive rounds of mutation and selection / Hayato Yanagida, Tomoaki Matsuura, and Tetsuya Yomo
Fine-tuning enzyme activity through saturation mutagenesis / Holly H. Hogrefe
Characterization of structural determinants of type 1 corticotropin releasing hormone (CRH) receptor signalling properties / Danijela Markovic and Dimitris K. Grammatopoulos
Site-directed mutagenesis for improving biophysical properties of Vh domains / Mehdi Arbabi-Ghahroudi, Roger MacKenzie, and Jamshid Tanha
Phenotype based functional gene screening using retrovirus-mediated gene trapping in quasi-haploid RAW 264.7 cells / Sung Ouk Kim and Soon-Duck Ha
Site-directed disulfide cross-linking to probe conformational changes of a transporter during its functional cycle : Escherichia coli AcrB multidrug exporter as an example / Yumiko Takatsuka and Hiroshi Nikaido
Site-specific incorporation of extra components into RNA by transcription uusing unnatural base pair systems / Michiko Kimoto and Ichiro Hirao
Mutagen : a random mutagenesis method providing a complementary diversity generated by human error-prone DNA polymerases / Philippe Mondon ... [et al.]
Random-scanning mutagenesis / Robert A. Smith
Easy two-step method for randomizing and cloning gene fragments / Vivan Q. Zhang and Holly H. Hogrefe
Random mutagenesis using a mutator strain / Ghazala Muteeb and Ranjan Sen
En passant mutagenesis : a two step markerless red recombination system / B. Karsten Tischer, Gregory A. Smith, and Nikolaus Osterrieder.
Show 27 more Contents items
ISBN
9781607616528
1607616521
OCLC
664248091
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In Vitro Mutagenesis Protocols [electronic resource] : Third Edition / edited by Jeff Braman.
id
99125110050106421
In vitro mutagenesis protocols / edited by Jeff Braman.
id
9963480233506421