In vitro mutagenesis protocols [electronic resource] / edited by Jeff Braman.

Format
Book
Language
English
Εdition
3rd ed.
Published/​Created
[Totowa, N.J.] : Humana Press, c2010.
Description
xiv, 442 p. : ill.

Details

Subject(s)
Series
Bibliographic references
Includes bibliographical references and index.
Reproduction note
Electronic reproduction. New York : Springer, 2010. Mode of access: World Wide Web. System requirements: Web browser. Title from title screen (viewed on Sep. 9, 2010). Access may be restricted to users at subscribing institutions.
Contents
  • Mutagenesis protocols in Saccharomyces cerevisiae by in vivo overlap extension / Mibuel Alcalde
  • In vitro mutagenesis of Brucella species / Thomas A. Ficht, Jianwu Pei, and Melissa Kahl-McDonagh
  • Random mutagenesis strategies for Campylobacter and Helicobacter species / Duncan J.H. Gaskin and Arnoud H.M. van Vliet
  • Mutagenesis of the repeat regions of herpesviruses cloned as bacterial artificial chromosomes / Yuguang Zhao and Venugopal Nair
  • An efficient protocol for VZV BAC-based mutagenesis / Zhen Zhang, Ying Huang, and Hua Zhu
  • A method for rapid genetic integration into Plasmodium falciparum utilizing mycobacteriophage Bxb1 integrase / Sophie H. Adjalley, Marcus C.S. Lee, and David A. Fidock
  • Random mutagenesis by error-prone PCR / Elizabeth O. McCullum ... [et al.]
  • A rapid and versatile PCR-based site-directed mutagenesis protocol for generation of mutations along the entire length of a cloned cDNA / Vincent Dammai
  • Rapid sequence scanning mutagenesis using in silico oligo design and the megaprimer PCR of whole plasmid method (MegaWhop) / Urich Krauss, Karl-Erich Jaeger, and Thorsten Eggert
  • Insertion and deletion mutagenesis by overlap extension PCR / Jehan Lee ... [et al.]
  • Targeted amplification of mutant strands for efficient site-directed mutagenesis and mutant screening / Lei Young and Qihan Dong
  • A modified inverse PCR procedure for insertion, deletion, or replacement of a DNA fragment in a target sequence and its application in the ligand interaction scan method for generation of ligand-regulated proteins / Oran Erster and Moti Liscovitch
  • Amplification of orthologous genes using degererate primers / Samya Chakravorty and jim O. Vigoreaux
  • Computational evaluation of protein stability change upon mutations / Shuangye Yin, Feng Ding, and Nikolay V. Dokholyan
  • Approaches for using animal models to identify loci that genetically interact with human disease-causing point mutations / Josef D. Franke
  • Using peptide loop insertion mutagenesis for the evolution of proteins / Christian Heinis and Kai Johnsson
  • Massive mutagenesis : high-throughput combinatorial site-directed mutagenesis / Julien Sylvestre
  • Directed in vitro evolution of reporter genes based on semi-rational design and high-throughput screening / Ai-Sheng Xiong ... [et al.]
  • Ribosome display for rapid protein evolution by consecutive rounds of mutation and selection / Hayato Yanagida, Tomoaki Matsuura, and Tetsuya Yomo
  • Fine-tuning enzyme activity through saturation mutagenesis / Holly H. Hogrefe
  • Characterization of structural determinants of type 1 corticotropin releasing hormone (CRH) receptor signalling properties / Danijela Markovic and Dimitris K. Grammatopoulos
  • Site-directed mutagenesis for improving biophysical properties of Vh domains / Mehdi Arbabi-Ghahroudi, Roger MacKenzie, and Jamshid Tanha
  • Phenotype based functional gene screening using retrovirus-mediated gene trapping in quasi-haploid RAW 264.7 cells / Sung Ouk Kim and Soon-Duck Ha
  • Site-directed disulfide cross-linking to probe conformational changes of a transporter during its functional cycle : Escherichia coli AcrB multidrug exporter as an example / Yumiko Takatsuka and Hiroshi Nikaido
  • Site-specific incorporation of extra components into RNA by transcription uusing unnatural base pair systems / Michiko Kimoto and Ichiro Hirao
  • Mutagen : a random mutagenesis method providing a complementary diversity generated by human error-prone DNA polymerases / Philippe Mondon ... [et al.]
  • Random-scanning mutagenesis / Robert A. Smith
  • Easy two-step method for randomizing and cloning gene fragments / Vivan Q. Zhang and Holly H. Hogrefe
  • Random mutagenesis using a mutator strain / Ghazala Muteeb and Ranjan Sen
  • En passant mutagenesis : a two step markerless red recombination system / B. Karsten Tischer, Gregory A. Smith, and Nikolaus Osterrieder.
ISBN
  • 9781607616528
  • 1607616521
OCLC
664248091
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