Cyclooxygenases [electronic resource] : methods and protocols / edited by Samir S. Ayoub, Roderick J. Flower, Michael P. Seed.

Format
Book
Language
English
Published/​Created
New York, NY : Humana Press ; Springer Science+Business Media, c2010.
Description
x, 218 p. : ill. ; 27 cm.

Details

Subject(s)
Series
Bibliographic references
Includes bibliographical references and index.
Reproduction note
Electronic reproduction. New York : Springer, 2010. Mode of access: World Wide Web. System requirements: Web browser. Title from title screen (viewed on Aug. 9, 2010). Access may be restricted to users at subscribing institutions.
Contents
  • Part I. In vitro protocols for studying expression and activity of cyclooxygenase enzymes and characterisation of their inhibition patterns
  • 1. In vitro cyclo-oxygenase expression and activity protocols : introduction to Part I / Roderick J. Flower
  • 2. Techniques used to study regulation of cyclooxygenase-2 promoter sites / Hiroyasu Inoue and Rieko Nakata
  • 3. Purification of recombinant human COX-1 and COX-2 / James K. Gierse
  • 4. Cloning and expression of cyclooxygenase-1 and cyclooxygenase-2 / Nicholas R. Staten and Beverly A. Reitz
  • 5. Expression of cyclooxygenase isoforms in the baculovirus expression system / John C. Hunter, Natalie M. Myres, and Daniel L. Simmons
  • 6. Peroxidase active site activity assay / Kelsey C. Duggan, Joel Musee, and Lawrence J. Marnett
  • 7. Study of inhibitors of the PGH synthases for which potency Is regulated by the redox state of the enzymes / Olivier Boutaud and John A. Oates
  • 8. Different methods for testing potential cyclooxygenase-1 and cyclooxygenase-2 inhibitors / Stefan Laufer and Sabine Luik
  • 9. In vitro cyclooxygenase activity assay in tissue homogenates / Samir S. Ayoub
  • 10. Techniques used to characterize the binding of cyclooxygenase Inhibitors to the cyclooxygenase active site / William F. Hood
  • 11. In vitro Assays for cyclooxygenase activity and inhibitor characterization / Mark C. Walker and James K. Gierse
  • -- Part II. Extraction and measurement of prostanoids and isoprostanes
  • 12. Extraction and measurement of prostanoids and isoprostanes : introduction to Part II / Paola Patrignani
  • 13. Prostanoid extraction and measurement / Lorenzo Polenzani and Samir S. Ayoub
  • 14. Measurement of 8-iso-prostaglandin F2a in biological fluids as a measure of lipid peroxidation / Stefania Tacconelli, Marta L. Capone, and Paola Patrignani
  • -- Part III. In vivo models to study involvement of cyclooxygenase products in health and disease
  • 15. In vivo models to study cyclooxygenase products in health and disease: introduction to Part III / Derek W. Gilroy, Melanie Stables, and Justine Newson
  • 16. Protocols to assess the gastrointestinal side effects resulting from inhibition of Cyclo-Oxygenase isoforms / Brendan J.R. Whittle
  • 17. Cyclooxygenase enzymes and their products in the carrageenan-induced pleurisy in rats / Adrian R. Moore, Samir S. Ayoub, and Michael P. Seed
  • 18. Iloprost-induced nociception : determination of the site of anti-nociceptive action of cyclooxygenase inhibitors and the Involvement of cyclooxygenase products in central mechanisms of nociception / Samir S. Ayoub and Regina M. Botting.
ISBN
  • 9781597453646
  • 1597453641
OCLC
658898966
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