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Cyclooxygenases [electronic resource] : methods and protocols / edited by Samir S. Ayoub, Roderick J. Flower, Michael P. Seed.
Format
Book
Language
English
Published/Created
New York, NY : Humana Press ; Springer Science+Business Media, c2010.
Description
x, 218 p. : ill. ; 27 cm.
Availability
Available Online
Online Content
SpringerNature Complete Protocols
Online Content
Details
Subject(s)
Cyclooxygenases
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Related name
Ayoub, Samir S.
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Flower, R. J. (Rod J.), 1945-
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Seed, Michael P.
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SpringerLink (Online service)
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Series
Methods in molecular biology (Clifton, N.J.) ; v. 644.
[More in this series]
Springer protocols.
[More in this series]
Methods in molecular biology, 1064-3745 ; 644
Bibliographic references
Includes bibliographical references and index.
Reproduction note
Electronic reproduction. New York : Springer, 2010. Mode of access: World Wide Web. System requirements: Web browser. Title from title screen (viewed on Aug. 9, 2010). Access may be restricted to users at subscribing institutions.
Contents
Part I. In vitro protocols for studying expression and activity of cyclooxygenase enzymes and characterisation of their inhibition patterns
1. In vitro cyclo-oxygenase expression and activity protocols : introduction to Part I / Roderick J. Flower
2. Techniques used to study regulation of cyclooxygenase-2 promoter sites / Hiroyasu Inoue and Rieko Nakata
3. Purification of recombinant human COX-1 and COX-2 / James K. Gierse
4. Cloning and expression of cyclooxygenase-1 and cyclooxygenase-2 / Nicholas R. Staten and Beverly A. Reitz
5. Expression of cyclooxygenase isoforms in the baculovirus expression system / John C. Hunter, Natalie M. Myres, and Daniel L. Simmons
6. Peroxidase active site activity assay / Kelsey C. Duggan, Joel Musee, and Lawrence J. Marnett
7. Study of inhibitors of the PGH synthases for which potency Is regulated by the redox state of the enzymes / Olivier Boutaud and John A. Oates
8. Different methods for testing potential cyclooxygenase-1 and cyclooxygenase-2 inhibitors / Stefan Laufer and Sabine Luik
9. In vitro cyclooxygenase activity assay in tissue homogenates / Samir S. Ayoub
10. Techniques used to characterize the binding of cyclooxygenase Inhibitors to the cyclooxygenase active site / William F. Hood
11. In vitro Assays for cyclooxygenase activity and inhibitor characterization / Mark C. Walker and James K. Gierse
-- Part II. Extraction and measurement of prostanoids and isoprostanes
12. Extraction and measurement of prostanoids and isoprostanes : introduction to Part II / Paola Patrignani
13. Prostanoid extraction and measurement / Lorenzo Polenzani and Samir S. Ayoub
14. Measurement of 8-iso-prostaglandin F2a in biological fluids as a measure of lipid peroxidation / Stefania Tacconelli, Marta L. Capone, and Paola Patrignani
-- Part III. In vivo models to study involvement of cyclooxygenase products in health and disease
15. In vivo models to study cyclooxygenase products in health and disease: introduction to Part III / Derek W. Gilroy, Melanie Stables, and Justine Newson
16. Protocols to assess the gastrointestinal side effects resulting from inhibition of Cyclo-Oxygenase isoforms / Brendan J.R. Whittle
17. Cyclooxygenase enzymes and their products in the carrageenan-induced pleurisy in rats / Adrian R. Moore, Samir S. Ayoub, and Michael P. Seed
18. Iloprost-induced nociception : determination of the site of anti-nociceptive action of cyclooxygenase inhibitors and the Involvement of cyclooxygenase products in central mechanisms of nociception / Samir S. Ayoub and Regina M. Botting.
Show 18 more Contents items
ISBN
9781597453646
1597453641
OCLC
658898966
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Cyclooxygenases [electronic resource] : Methods and Protocols / edited by Samir S. Ayoub, Roderick J. Flower, Michael Seed.
id
99125201985406421
Cyclooxygenases : methods and protocols / edited by Samir S. Ayoub, Roderick J. Flower, Michael P. Seed.
id
9964690973506421