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Immunoelectron microscopy : methods and protocols / edited by Steven D. Schwartzbach, Testuaki Osafune.
Format
Book
Language
English
Published/Created
New York : Humana Press, ©2010.
Description
xi, 352 pages : illustrations
Availability
Available Online
Online Content
SpringerNature Complete Protocols
Online Content
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Status
Location Service
Notes
Lewis Library - Lewis Library - Serials (Off-Site)
QH506 .M45 1984 vol.657
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Subject(s)
Electron microscopy
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Technique
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Related name
Schwartzbach, Steven D.
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Osafune, Tetsuaki
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Series
Methods in molecular biology (Clifton, N.J.) ; v. 657.
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Springer protocols (Series)
[More in this series]
Methods in molecular biology, 1064-3745 ; 657
Springer protocols
Summary note
Immunoelectron microscopy is a key technique that bridges the information gap between biochemistry, molecular biology, and ultrastructural studies placing macromolecular functions within a cellular context. In Immunoelectron Microscopy: Methods and Protocols, expert researchers combine the tools of the molecular biologist with those of the microscopist. From the molecular biology toolbox, this volume presents methods for antigen production by protein expression in bacterial cells, methods for epitope tagged protein expression in plant and animal cells allowing protein localization in the absence of protein specific antibodies as well as methods for the production of anti-peptide, monoclonal, and polyclonal antibodies. From the microscopy toolbox, sample preparation methods for cells, plant, and animal tissue are presented. Both cryo-methods, which have the advantage of retaining protein antigenicity at the expense of ultrastructural integrity, as well as chemical fixation methods that maintain structural integrity while sacrificing protein antigenicity have been included, with chapters examining various aspects of immunogold labeling. Written in the highly successful Methods in Molecular Biology" series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls
Authoritative and essential, Immunoelectron Microscopy: Methods and Protocols seeks to facilitate an increased understanding of structure function relationships --Book Jacket.
Bibliographic references
Includes bibliographical references and index.
Contents
Protein antigen expression in Escherichia coli for antibody production / David M. Rancour, Steven K. Backues, and Sebastian Y. Bednarek
Expression of epitope-tagged proteins in plants / Takuya Furuichi
Expression of epitope-tagged proteins in Arabidopsis leaf mesophyll protoplasts / Young-Hee Cho and Sang-Dong Yoo
Transient expression of epitope-tagged proteins in mammalian cells / Melanie L. Styers, Jason Lowery, and Elizabeth Sztul
Production and purification of polyclonal antibodies / Masami Nakazawa, Mari Mukumoto, and Kazutaka Miyatake
Production and purification of monoclonal antibodies / Masami Nakazawa, Mari Mukumoto, and Kazutaka Miyatake
Production of antipeptide antibodies / Bao-Shiang Lee ... [et al.]
Preparation of colloidal gold particles and conjugation to protein A, IgG, F(ab')₂, and streptavidin / Sadaki Yokota
Immunoelectron microscopy of chemically fixed developing plant embryos / Tetsuaki Osafune and Steven D. Schwartzbach
Pre-embedding immunogold localization of antigens in mammalian brain slices / Thomas Schikorski
Pre-embedding immunoelectron microscopy of chemically fixed mammalian tissue culture cells / Haruo Hagiwara ... [et al.]
Immunoelectron microscopy of cryofixed and freeze-substituted plant tissues / Miyuki Takeuchi, Keiji Takabe, and Yoshinobu Mineyuki
In vivo cryotechniques for preparation of animal tissues for immunoelectron microscopy / Shinichi Ohno ... [et al.]
Immunoelectron microscopy of cryofixed freeze-substituted mammalian tissue culture cells / Akira Sawaguchi
Immunoelectron microscopy of cryofixed freeze-substituted Saccharomyces cerevisiae / Jindriska Fiserova and Martin W. Goldberg
High-resolution molecular localization by freeze-fracture replica labeling / Akikazu Fujita and Toyoshi Fujimoto
Pre-embedding electron microscopy methods for glycan localization in chemically fixed mammalian tissue using horseradish peroxidase-conjugated lectin / Yoshihiro Akimoto and Hayato Kawakami
Pre-embedding nanogold silver and gold intensification / Akitsugu Yamamoto and Ryuichi Masaki
The post-embedding method for immunoelectron microscopy of mammalian tissues : a standardized procedure based on heat-induced antigen retrieval / Shuji Yamashita
Double-label immunoelectron microscopy for studying the colocalization of proteins in cultured cells / Haruo Hagiwara ... [et al.]
Serial section immunoelectron microscopy of algal cells / Tetsuaki Osafune and Steven D. Schwartzbach
Freeze-etch electron tomography for the plasma membrane interface / Nobuhiro Morone
Localization of rDNA at nucleolar structural components by immunoelectron microscopy / Seiichi Sato and Yasushi Sato
Immunogold labelling for scanning electron microscopy / Martin W. Goldberg and Jindriska Fiserova
Horseradish peroxidase as a reporter gene and as a cell-organelle-specific marker in correlative light-electron microscopy / Thomas Schikorski
Monitoring rapid endocytosis in the electron microscope via photoconversion of vesicles fluorescently labeled with FM1-43 / Thomas Schikorski.
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Other format(s)
Also available in an electronic version.
ISBN
9781607617822 ((alk. paper))
160761782X ((alk. paper))
9781607617839 ((e-ISBN))
1607617838 ((e-ISBN))
LCCN
2010929610
OCLC
639187711
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Immunoelectron Microscopy [electronic resource] : Methods and Protocols / edited by Steven D. Schwartzbach, Tetsuaki Osafune.
id
99125187203806421
Immunoelectron microscopy [electronic resource] : methods and protocols / edited by Steven L. Schwartzbach, Testuaki Osafune.
id
9962473533506421