Plant epigenetics : methods and protocols / edited by Igor Kovalchuk, Franz J. Zemp.

Format
Book
Language
English
Published/​Created
New York : Humana Press, ©2010.
Description
x, 273 pages : illustrations

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Lewis Library - Lewis Library - Serials (Off-Site) QH506 .M45 1984 vol.631 Browse related items Request

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    Summary note
    The past fifteen years have witnessed major advances in epigenetics, one of the most popular and quickly evolving fields of modern science. In Plant Epigenetics: Methods and Protocols, expert researchers explore the most recent developments, examining in great detail the contribution of epigenetic regulation to cell function in plants. Chapters include a variety of protocols for studying the function of small non-coding RNAs, DNA methylation, and histone modifications in plants, often in different degrees of complexity. This volume describes bioinformatic approaches to the analysis of high-throughput data, such as bisulfite sequencing and Chip-on-chip assays. It features much-desired protocols for plant transgenesis and the analysis of genome stability, with a detailed discussion of their applications to epigenetic studies. Composed in the highly successful Methods in Molecular Biologyâ„¢ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.
    Bibliographic references
    Includes bibliographical references and index.
    Contents
    • Analysis of DNA methylation in plants by bisulfite sequencing / Andrea Foerster and Ortrun Mittelsten Scheid
    • Analysis of bisulfite sequencing data from plant DNA using CyMATE / Andrea M. Foerster [and others]
    • Analysis of locus-specific changes in methylation patterns using a COBRA (combined bisulfite restriction analysis) assay / Alex Boyko and Igor Kovalchuk
    • Detection of changes in global genome methylation using the cytosine-extension assay / Alex Boyko and Igor Kovalchuk
    • In situ analysis of DNA methylation in plants / Palak Kathiria and Igor Kovalchuk
    • Analysis of mutation/rearrangement frequencies and methylation patterns at a given DNA locus using restriction fragment length polymorphism / Alex Boyko and Igor Kovalchuk
    • Isoschizomers and amplified fragment length polymorphism for the detection of specific cytosine methylation changes / Leonor Ruiz-García [and others]
    • Analysis of small RNA populations using hybridization to DNA tiling arrays / Martine Boccara [and others]
    • Northern blotting techniques for small RNAs / Todd Blevins
    • qRT-PCR of small RNAs / Erika Varkonyi-Gasic and Roger P. Hellens
    • Cloning new small RNA sequences / Yuko Tagami, Naoko Inaba, and Yuichiro Watanabe
    • Genome-wide mapping of protein-DNA interaction by chromatin immunoprecipitatino and DNA microarray hybridization (ChIP-chip). Part A : ChiP-chip molecular methods / Julia J. Reimer and Franziska Turck. Part B : ChIP-chip data analysis / Ulrike Göbel, Julia Reimer, and Franziska Turck
    • Metaanalysis of ChIP-chip data / Julia Engelhorn and Franziska Turck
    • Chromatin immunoprecipitation protocol for histone modifications and protein-DNA binding analyses in Arabidopsis / Stéphane Pien and Ueli Grossniklaus
    • cDNA libraries for virus-induced gene silencing / Andrea T. Todd, Enwu Liu, and Jonathan E. Page
    • Detection and quantification of DNA strand breaks using the ROPS (random oligonucleotide primed synthesis) assay / Alex Boyko and Igor Kovalchuk
    • Reporter gene-based recombination lines for studies of genome stability / Palak Kathiria and Igor Kovalchuk
    • Plant transgenesis / Alicja Ziemienowicz.
    Other format(s)
    Also available in an electronic version.
    ISBN
    • 9781607616450 ((alk. paper))
    • 1607616459 ((alk. paper))
    • 9781607616467 ((e-ISBN))
    • 1607616467 ((e-ISBN))
    LCCN
    2010922364
    OCLC
    471801564
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