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Bacterial protein secretion systems : methods and protocols / editors Laure Journet and Eric Cascales.
Format
Book
Language
English
Published/Created
New York, NY : Humana Press ; Springer, c2017.
Description
xv, 528 pages : illustrations (some color).
Availability
Available Online
Online Content
Springer Nature - Complete Protocols
Copies in the Library
Location
Call Number
Status
Location Service
Notes
ReCAP - Remote Storage
QH506 M45 1984 vol.1615
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Details
Subject(s)
Bacterial proteins
—
Laboratory manuals
[Browse]
Editor
Journet, Laure
[Browse]
Cascales, Eric
[Browse]
Library of Congress genre(s)
Laboratory manuals
[Browse]
Series
Methods in molecular biology (Clifton, N.J.) ; v. 1615.
[More in this series]
Methods in molecular biology, 1064-3745 ; v. 1615
Bibliographic references
Includes bibliographical references and index.
Source of description
Online resource; title from PDF title page (SpringerProtocol, viewed July 5, 2017).
Contents
Identification of protein secretion systems in bacterial genomes using MacSyFinder / Sophie S. Abby and Eduardo P.C. Rocha
Protein sorting prediction / Henrik Nielsen
Cell fractionation / Melissa Petiti, Laetitia Houot, and Denis Duché
Defining lipoprotein localisation by fluorescence microscopy / Maria Guillermina Casabona, Mylène Robert-Genthon, Didier Grunwald, and Ina Attrée
Identification of lipoproteins using globomycin and radioactive palmitate / Nienke Buddelmeijer
Defining membrane protein localization by isopycnic density gradients / Rhys A. Dunstan, Iain D. Hay, and Trevor Lithgow
Cell surface exposure / Anna Konovalova
Probing inner membrane protein topology by proteolysis / Maxence S. Vincent and Eric Cascales
Mapping of membrane protein topology by substituted cysteine accessibility method (SCAM) / Mikhail Bogdanov
Defining membrane protein topology using pho-lac reporter fusions / Gouzel Karimova and Daniel Ladant
In vivo and in vitro protein-peptidoglycan interactions / Gang Li and S. Peter Howard
Measure of peptidoglycan hydrolase activity / Yoann G. Santin and Eric Cascales
Protein-protein interaction : Bacterial Two-Hybrid / Gouzel Karimova, Emilie Gauliard, Marilyne Davi, Scot P. Ouellette, and Daniel Ladant
Protein-protein interactions : yeast two-hybrid system / Jer-Sheng Lin and Erh-Min Lai
Protein-protein interactions : cytology two-hybrid / Krishnamohan Atmakuri
Fusion reporter approaches to monitoring transmembrane helix interactions in bacterial membranes / Laureen Logger, Abdelrahim Zoued, and Eric Cascales
Protein-protein interactions : co-immunoprecipitation / Jer-Sheng Lin and Erh-Min Lai
Protein-protein interaction : tandem affinity purification in bacteria / Julie P.M. Viala and Emmanuelle Bouveret
Site-directed and time-resolved photocrosslinking in cells metabolically labeled with radioisotopes / Raffaele Ieva
Protein-protein interactions : pull-down assays / Arthur Louche, Suzana P. Salcedo, and Sarah Bigot
Protein-protein interactions : surface plasmon resonance / Badreddine Douzi
Assessing energy-dependent protein conformational changes in the TonB system / Ray A. Larsen
Defining assembly pathways by fluorescence microscopy / Abdelrahim Zoued and Andreas Diepold
Large complexes : cloning strategy, production, and purification / Eric Durand and Roland Lloubes
Shearing and enrichment of extracellular type IV pili / Alba Katiria Gonzalez Rivera and Katrina T. Forest
Blue native PAGE analysis of bacterial secretion complexes / Susann Zilkenat, Tobias Dietsche, Julia V. Monjarás Feria, Claudia E. Torres-Vargas, Mehari Tesfazgi Mebrhatu, and Samuel Wagner
In situ imaging of bacterial secretion systems by electron cryotomography / Gregor L. Weiss, João M. Medeiros, and Martin Pilhofer
Structural analysis of protein complexes by cryo electron microscopy / Tiago R.D. Costa, Athanasios Ignatiou, and Elena V. Orlova
Bacterial filamentous appendages investigated by solid-state NMR spectroscopy / Birgit Habenstein and Antoine Loquet
Energy requirements for protein secretion via the flagellar type III secretion system / Marc Erhardt
Identification of effectors : precipitation of supernatant material / Nicolas Flaugnatti and Laure Journet
Screening for secretion of the type VI secretion system protein Hcp by enzyme-linked immunosorbent assay and colony blot / Brent S. Weber, Pek Man Ly, and Mario F. Feldman
Effector translocation : cya reporter assay / Suma Chakravarthy, Bethany Huot, and Brian H. Kvitko
Monitoring effector translocation using the TEM-1 beta-lactamase reporter system / Julie Allombert, Anne Vianney, and Xavier Charpentier
Effector translocation assay : differential solubilization / Irina S. Franco, Sara V. Pais, Nuno Charro, and Luís Jaime Mota
Quantitative determination of anti-bacterial activity during bacterial co-culture / Juliana Alcoforado Diniz, Birte Hollmann, and Sarah J. Coulthurst.
Show 33 more Contents items
Other format(s)
Also available in an electronic version.
ISBN
9781493970339 ((electronic bk.))
149397033X ((electronic bk.))
OCLC
1171465708
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Bacterial Protein Secretion Systems [electronic resource] : Methods and Protocols / edited by Laure Journet, Eric Cascales.
id
99125108706606421