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Isolation of plant organelles and structures : methods and protocols / edited by Nicolas L. Taylor, A. Harvey Millar.
Format
Book
Language
English
Published/Created
New York, NY : Humana Press ; Springer, [2017]
©2017
Description
1 online resource (xi, 338 pages) : illustrations (some color).
Availability
Available Online
Springer Nature - Complete Protocols
Copies in the Library
Location
Call Number
Status
Location Service
Notes
Lewis Library - Lewis Library - Serials (Off-Site)
QH506 .M45 1984 vol.1511
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Details
Subject(s)
Plant organelles
—
Laboratory manuals
[Browse]
Editor
Taylor, Nicolas L.
[Browse]
Millar, A. Harvey
[Browse]
Library of Congress genre(s)
Laboratory manuals
[Browse]
Series
Methods in molecular biology (Clifton, N.J.) ; v. 1511.
[More in this series]
Springer protocols (Series)
[More in this series]
Methods in molecular biology, 1940-6029 ; volume 1511
Springer protocols
Summary note
This book brings together the major techniques used in the isolation or enrichment of individual populations of organelles and other subcellular structures from plants with the goal that, by being able to isolate subcellular structures, the research and understanding of various facets of compartmentalized function in plant cells can be advanced. Written for the highly successful Methods in Molecular Biology series, expert contributors provide chapters that contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Isolation of Plant Organelles and Structures: Methods and Protocols will greatly aid those who regularly isolate subcellular components as well as those whose research has lead them to focus on a subcellular compartment or a particular process for the first time, thus producing the need to be able to isolate it or enrich it for study.
Bibliographic references
Includes bibliographical references and index.
Source of description
Online resource; title from PDF title page (SpringerProtocol, viewed October 14, 2016).
Contents
Isolation of plant organelles and structures in the post-genomic era / A. Harvey Millar and Nicolas L. Taylor
Approaches to characterize organelle, compartment, or structure purity / Stefanie J. Mueller, Sebastian N.W. Hoernstein, and Ralf Reski
Isolation of nuclei and nucleoli / Alison F. Pendle and Peter J. Shaw
Isolation and suborganellar fractionation of Arabidopsis chloroplasts / Úrsula Flores-Pérez and Paul Jarvis
Isolation of chromoplasts and suborganellar compartments from tomato and bell pepper fruit / Cristina Barsan, Marcel Kuntz, and Jean-Claude Pech
Leucoplast isolation and subfractionation / William C. Plaxton
Isolation of mitochondria, their sub-organellar compartments, and membranes / Owen Duncan, A. Harvey Millar, and Nicolas L. Taylor
Isolation of Arabidopsis leaf peroxisomes and the peroxisomal membrane / Sigrun Reumann and Piotr Lisik
Isolation of vacuoles and the tonoplast / Jan Zouhar
Isolation of endoplasmic reticulum and its membrane / G. Eric Schaller
Enrichment of golgi membranes from Triticum aestivum (wheat) seedlings / Wei Zeng ... [et al.]
Isolation of autolysosomes from tobacco BY-2 cells / Chihiro Takatsuka, Yuko Inoue-Aono, and Yuji Moriyasu
Isolation of protein storage vacuoles and their membranes / Tomoo Shimada and Ikuko Hara-Nishimura
Isolation of the cell wall / Hervé Canut, Cécile Albenne, and Elisabeth Jamet
Isolation of plasmodesmata / Christine Faulkner and Emmanuelle M.F. Bayer
Isolation of plasma membrane and plasma membrane microdomains / Anzu Minami ... [et al.]
Enrichment of the plant cytosolic fraction / Jeemeng Lao ... [et al.]
Isolation of apoplast / Erik Andreasson, Kibrom B. Abreha, and Svante Resjö
Isolation of cytosolic ribosomes / Hanna Klang Årstrand and Maria V. Turkina
Isolation of plastid ribosomes / Kenichi Yamaguchi
Isolation of mitochondrial ribosomes / Adam J. Carroll
Isolation of microtubules and microtubule-associated proteins / Takahiro Hamada and Seiji Sonobe
Isolation of actin and actin-binding proteins / Etsuo Yokota
Purification of 26S proteasomes and their subcomplexes from plants / Richard S. Marshall, David C. Gemperline, and Richard D. Vierstra.
Show 21 more Contents items
ISBN
9781493965335 ((electronic bk.))
1493965336 ((electronic bk.))
OCLC
960724790
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Princeton University Library aims to describe library materials in a manner that is respectful to the individuals and communities who create, use, and are represented in the collections we manage.
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Isolation of Plant Organelles and Structures [electronic resource] : Methods and Protocols / edited by Nicolas L. Taylor, A. Harvey Millar.
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99125326368206421